• Immunohistochemistry analysis of paraffin-embedded rat heart using AKR1C3 antibody (STJ115529) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using AKR1C3 antibody (STJ115529) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AKR1C3 antibody (1-323) (STJ115529)

SKU:
STJ115529

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AKR1C3 (1-323) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKR1C3
Gene ID: 8644
Uniprot ID: AK1C3_HUMAN
Immunogen Region: 1-323
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C3 (NP_003730.4).
Immunogen Sequence: MDSKHQCVKLNDGHFMPVLG FGTYAPPEVPRSKALEVTKL AIEAGFRHIDSAHLYNNEEQ VGLAIRSKIADGSVKREDIF YTSKLWSTFHRPELVRPALE NSLKKAQLDYVDLYLIHSPM SLKPGEELSPTDENGKVIFD IVDLCTTWEAMEKCKDAGLA KSIGVSNFNRRQLEMILNKP GLKYKPVCNQVECHPYFNRS KLLDFCKSKDIVLVAYSALG SQRDKRWVDPNSPVLLEDP
Tissue Specificity Expressed in many tissues including adrenal gland, brain, kidney, liver, lung, mammary gland, placenta, small intestine, colon, spleen, prostate and testis. High expression in prostate and mammary gland. In the prostate, higher levels in epithelial cells than in stromal cells. In the brain, expressed in medulla, spinal cord, frontotemporal lobes, thalamus, subthalamic nuclei and amygdala. Weaker expression in the hippocampus, substantia nigra and caudate.
Function Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Acts as a NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain and regulates the metabolism of androgens, estrogens and progesterone. Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH. Acts preferentially as a 17-ketosteroid reductase and has the highest catalytic efficiency of the AKR1C enzyme for the reduction of delta4-androstenedione to form testosterone. Reduces prostaglandin (PG) D2 to 11beta-prostaglandin F2, progesterone to 20alpha-hydroxyprogesterone and estrone to 17beta-estradiol. Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Also displays retinaldehyde reductase activity toward 9-cis-retinal.
Protein Name Aldo-Keto Reductase Family 1 Member C3
17-Beta-Hydroxysteroid Dehydrogenase Type 5
17-Beta-Hsd 5
3-Alpha-Hsd Type Ii - Brain
3-Alpha-Hydroxysteroid Dehydrogenase Type 2
3-Alpha-Hsd Type 2
Chlordecone Reductase Homolog Hakrb
Dihydrodiol Dehydrogenase 3
Dd-3
Dd3
Dihydrodiol Dehydrogenase Type I
Ha1753
Prostaglandin F Synthase
Pgfs
Testosterone 17-Beta-Dehydrogenase 5
Database Links Reactome: R-HSA-193368
Reactome: R-HSA-193775
Reactome: R-HSA-193807
Reactome: R-HSA-2162123
Reactome: R-HSA-5365859
Reactome: R-HSA-975634
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Aldo-Keto Reductase Family 1 Member C3 antibody
Anti-17-Beta-Hydroxysteroid Dehydrogenase Type 5 antibody
Anti-17-Beta-Hsd 5 antibody
Anti-3-Alpha-Hsd Type Ii - Brain antibody
Anti-3-Alpha-Hydroxysteroid Dehydrogenase Type 2 antibody
Anti-3-Alpha-Hsd Type 2 antibody
Anti-Chlordecone Reductase Homolog Hakrb antibody
Anti-Dihydrodiol Dehydrogenase 3 antibody
Anti-Dd-3 antibody
Anti-Dd3 antibody
Anti-Dihydrodiol Dehydrogenase Type I antibody
Anti-Ha1753 antibody
Anti-Prostaglandin F Synthase antibody
Anti-Pgfs antibody
Anti-Testosterone 17-Beta-Dehydrogenase 5 antibody
Anti-AKR1C3 antibody
Anti-DDH1 antibody
Anti-HSD17B5 antibody
Anti-KIAA0119 antibody
Anti-PGFS antibody

Information sourced from Uniprot.org

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