| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-AKR1B10 (1-316) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AKR1B10 |
| Gene ID: |
57016 |
| Uniprot ID: |
AK1BA_HUMAN |
| Immunogen Region: |
1-316 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human AKR1B10 (NP_064695.3). |
| Immunogen Sequence: |
MATFVELSTKAKMPIVGLGT WKSPLGKVKEAVKVAIDAGY RHIDCAYVYQNEHEVGEAIQ EKIQEKAVKREDLFIVSKLW PTFFERPLVRKAFEKTLKDL KLSYLDVYLIHWPQGFKSGD DLFPKDDKGNAIGGKATFLD AWEAMEELVDEGLVKALGVS NFSHFQIEKLLNKPGLKYKP VTNQVECHPYLTQEKLIQYC HSKGITVTAYSPLGSPDRPW AKPEDPSLLEDPKIKEIAA |
| Tissue Specificity | Found in many tissues. Highly expressed in small intestine, colon and adrenal gland. |
| Function | Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays strong enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Plays a critical role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls). Displays no reductase activity towards glucose. |
| Protein Name | Aldo-Keto Reductase Family 1 Member B10Arl-1Aldose Reductase-LikeAldose Reductase-Related ProteinArpHarpSmall Intestine ReductaseSi Reductase |
| Database Links | Reactome: R-HSA-975634 |
| Cellular Localisation | LysosomeSecretedSecreted Through A Lysosome-Mediated Non-Classical Pathway |
| Alternative Antibody Names | Anti-Aldo-Keto Reductase Family 1 Member B10 antibodyAnti-Arl-1 antibodyAnti-Aldose Reductase-Like antibodyAnti-Aldose Reductase-Related Protein antibodyAnti-Arp antibodyAnti-Harp antibodyAnti-Small Intestine Reductase antibodyAnti-Si Reductase antibodyAnti-AKR1B10 antibodyAnti-AKR1B11 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and AKR1B1 knockout (KO) HeLa cells, using [KO Validated] AKR1B1 Rabbit polyclonal antibody (STJ11100004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89077/357534/STJ11100004_1__87524.1713121649.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and AKR1B1 knockout (KO) HeLa cells, using [KO Validated] AKR1B1 Rabbit polyclonal antibody (STJ11100004) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
