• Confocal immunofluorescence analysis of U2OS cells using AKAP8 Rabbit polyclonal antibody (STJ22564) at dilution of 1:200. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using AKAP8 Rabbit polyclonal antibody (STJ22564) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-AKAP8 antibody (480-692) (STJ22564)

SKU:
STJ22564

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AKAP8 (480-692) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKAP8
Gene ID: 10270
Uniprot ID: AKAP8_HUMAN
Immunogen Region: 480-692
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 480-692 of human AKAP8 (NP_005849.1).
Immunogen Sequence: HCLACDMLIPAQPQLLQRHL HSVDHNHNRRLAAEQFKKTS LHVAKSVLNNRHIVKMLEKY LKGEDPFTSETVDPEMEGDD NLGGEDKKETPEEVAADVLA EVITAAVRAVDGEGAPAPES SGEPAEDEGPTDTAEAGSDP QAEQLLEEQVPCGTAHEKGV PKARSEAAEAGNGAETMAAE AESAQTRVAPAPAAADAEVE QTDAESKDAVPTE
Tissue Specificity Highly expressed in heart, liver, skeletal muscle, kidney and pancreas. Expressed in mature dendritic cells.
Post Translational Modifications Phosphorylated on tyrosine residues probably by SRC subfamily protein kinases.multiple phosphorylation is leading to dissociation from nuclear structures implicated in chromatin structural changes.
Function Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation.the function appears to be independent from PKA-anchoring. May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3.in this function may act redundantly with AKAP8L. Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation. May be involved in regulation of DNA replication by acting as scaffold for MCM2. Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation. May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells. May act as a carrier protein of GJA1 for its transport to the nucleus. May play a repressive role in the regulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro. In cells, associates with ribosomal RNA (rRNA) chromatin, preferentially with rRNA promoter and transcribed regions. Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation.the function probably implicates targeting of PKA to NFKB1.
Protein Name A-Kinase Anchor Protein 8
Akap-8
A-Kinase Anchor Protein 95 Kda
Akap 95
Cellular Localisation Nucleus
Nucleus Matrix
Nucleolus
Cytoplasm
Associated With The Nuclear Matrix In Interphase And Redistributes Mostly To Chromatin At Mitosis
However
Mitotic Chromatin Localization Has Been Questioned
Upon Nuclear Reassembly At The End Of Mitosis
Is Sequestered Into The Daughter Nuclei Where It Re-Acquires An Interphase Distribution
Exhibits Partial Localization To The Nucleolus In Interphase
Where It Colocalizes With Ubtf/Ubf
Suggesting Localization To The Fibrillary Center And/Or To The Dense Fibrillary Component
Colocalizes With Gja1 At The Nuclear Membrane Specifically During Cell Cycle G1/S Phase
Alternative Antibody Names Anti-A-Kinase Anchor Protein 8 antibody
Anti-Akap-8 antibody
Anti-A-Kinase Anchor Protein 95 Kda antibody
Anti-Akap 95 antibody
Anti-AKAP8 antibody
Anti-AKAP95 antibody

Information sourced from Uniprot.org

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