Western blot analysis of extracts of various cell lines, using AKAP14 Rabbit monoclonal antibody (STJ11103158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-AKAP14 antibody (70-150) [S8MR] (STJ11103158)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11103158
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General Information

Short DescriptionRabbit monoclonal AKAP14 (70-150) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDS8MR
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolAKAP14
Gene ID158798
Uniprot IDAKA28_HUMAN
Immunogen Region70-150
Immunogen SequenceHGEFTVEKGLKQIDEYFSKC VSKKCWAHGVEFVERKDLIH SFLYIYYVHWSISTADLPVA RISAGTYFTMKVSKTKPPDA P
SpecificityA synthetic peptide corresponding to a sequence within amino acids 70-150 of human AKAP14 (Q86UN6).

Additional Info

Tissue Specificity Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia.
Function Binds to type II regulatory subunits of protein kinase A and anchors/targets them.
Protein Name A-Kinase Anchor Protein 14
Akap-14
A-Kinase Anchor Protein 28 Kda
Akap 28
Protein Kinase A-Anchoring Protein 14
Prka14
Database Links
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-A-Kinase Anchor Protein 14 antibody
Anti-Akap-14 antibody
Anti-A-Kinase Anchor Protein 28 Kda antibody
Anti-Akap 28 antibody
Anti-Protein Kinase A-Anchoring Protein 14 antibody
Anti-Prka14 antibody
Anti-AKAP14 antibody
Anti-AKAP28 antibody

Information sourced from Uniprot.org

Citations

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