• Western blot analysis of extracts of various cell lines, using AKAP14 Rabbit monoclonal antibody (STJ11103158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-AKAP14 antibody (70-150) [S8MR] (STJ11103158)

SKU:
STJ11103158

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-AKAP14 (70-150) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AKAP14
Gene ID: 158798
Uniprot ID: AKA28_HUMAN
Immunogen Region: 70-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 70-150 of human AKAP14 (Q86UN6).
Immunogen Sequence: HGEFTVEKGLKQIDEYFSKC VSKKCWAHGVEFVERKDLIH SFLYIYYVHWSISTADLPVA RISAGTYFTMKVSKTKPPDA P
Tissue Specificity Present in cilia (at protein level). Expressed in tissues containing axoneme-based organelles (cilia and/or flagella): trachea and testis. Highly expressed in airway cilia.
Function Binds to type II regulatory subunits of protein kinase A and anchors/targets them.
Protein Name A-Kinase Anchor Protein 14
Akap-14
A-Kinase Anchor Protein 28 Kda
Akap 28
Protein Kinase A-Anchoring Protein 14
Prka14
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-A-Kinase Anchor Protein 14 antibody
Anti-Akap-14 antibody
Anti-A-Kinase Anchor Protein 28 Kda antibody
Anti-Akap 28 antibody
Anti-Protein Kinase A-Anchoring Protein 14 antibody
Anti-Prka14 antibody
Anti-AKAP14 antibody
Anti-AKAP28 antibody

Information sourced from Uniprot.org

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