• Immunohistochemistry analysis of AK5 in paraffin-embedded rat brain using AK5 Rabbit polyclonal antibody (STJ117619) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using AK5 Rabbit polyclonal antibody (STJ117619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-AK5 antibody (150-370) (STJ117619)

SKU:
STJ117619

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AK5 (150-370) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AK5
Gene ID: 26289
Uniprot ID: KAD5_HUMAN
Immunogen Region: 150-370
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 150-370 of human AK5 (NP_777283.1).
Immunogen Sequence: LKIAERYGFQYISVGELLRK KIHSTSSNRKWSLIAKIITT GELAPQETTITEIKQKLMQI PDEEGIVIDGFPRDVAQALS FEDQICTPDLVVFLACANQR LKERLLKRAEQQGRPDDNVK ATQRRLMNFKQNAAPLVKYF QEKGLIMTFDADRDEDEVFY DISMAVDNKLFPNKEAAAGS SDLDPSMILDTGEIIDTGSD YEDQGDDQLNVFGEDTMGGF M
Tissue Specificity Brain specific.
Function Nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Active on AMP and dAMP with ATP as a donor. When GTP is used as phosphate donor, the enzyme phosphorylates AMP, CMP, and to a small extent dCMP. Also displays broad nucleoside diphosphate kinase activity.
Protein Name Adenylate Kinase Isoenzyme 5
Ak 5
Atp-Amp Transphosphorylase 5
Database Links Reactome: R-HSA-499943
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Adenylate Kinase Isoenzyme 5 antibody
Anti-Ak 5 antibody
Anti-Atp-Amp Transphosphorylase 5 antibody
Anti-AK5 antibody

Information sourced from Uniprot.org

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