• Immunohistochemistry analysis of paraffin-embedded mouse heart using AK1 Antibody (STJ115421) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using AK1 Antibody (STJ115421) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat kidney using AK1 Antibody (STJ115421) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using AK1 antibody (STJ115421) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-AK1 antibody (1-194) (STJ115421)

SKU:
STJ115421

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AK1 (1-194) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AK1
Gene ID: 203
Uniprot ID: KAD1_HUMAN
Immunogen Region: 1-194
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-194 of human AK1 (NP_000467.1).
Immunogen Sequence: MEEKLKKTKIIFVVGGPGSG KGTQCEKIVQKYGYTHLSTG DLLRSEVSSGSARGKKLSEI MEKGQLVPLETVLDMLRDAM VAKVNTSKGFLIDGYPREVQ QGEEFERRIGQPTLLLYVDA GPETMTQRLLKRGETSGRVD DNEETIKKRLETYYKATEPV IAFYEKRGIVRKVNAEGSVD SVFSQVCTHLDALK
Function Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Exhibits nucleoside diphosphate kinase activity, catalyzing the production of ATP, CTP, GTP, UTP, dATP, dCTP, dGTP and dTTP from the corresponding diphosphate substrates with either ATP or GTP as phosphate donor. Also catalyzes at a very low rate the synthesis of thiamine triphosphate (ThTP) from thiamine diphosphate (ThDP) and ADP.
Protein Name Adenylate Kinase Isoenzyme 1
Ak 1
Atp-Amp Transphosphorylase 1
Atp -Amp Phosphotransferase
Adenylate Monophosphate Kinase
Myokinase
Database Links Reactome: R-HSA-499943
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Adenylate Kinase Isoenzyme 1 antibody
Anti-Ak 1 antibody
Anti-Atp-Amp Transphosphorylase 1 antibody
Anti-Atp -Amp Phosphotransferase antibody
Anti-Adenylate Monophosphate Kinase antibody
Anti-Myokinase antibody
Anti-AK1 antibody

Information sourced from Uniprot.org

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