• STJ70203 (1µg/ml) staining of nuclear Jurkat cell (A) , (2ug/ml) Human Spleen (B) and negative control Human Smooth Muscle (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70203 (2µg/ml) staining of paraffin embedded Human Thymus. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.
  • STJ70203 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70203 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nucleoplasm and nuclear membrane staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70203 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-AIRE antibody (C-Term) (STJ70203)

SKU:
STJ70203-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-AIRE (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-2µg/ml
IHC-2.5µg/ml
IF-Strong expression of the protein seen in the nucleoplasm and nuclear membranes of U2OS and HeLa cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: AIRE
Gene ID: 326
Uniprot ID: AIRE_HUMAN
Immunogen Region: C-Term
Accession Number: NP_000374.1
Immunogen Sequence: QSMARPAAPFPS
Post Translational Modifications Phosphorylated. Phosphorylation could trigger oligomerization.
Function Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA). Binds to G-doublets in an A/T-rich environment.the preferred motif is a tandem repeat of 5'-ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes. Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla. Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program. Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression. Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes. In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection. In secondary lymphoid organs, induces functional inactivation of CD4(+) T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli.
Protein Name Autoimmune Regulator
Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein
Apeced Protein
Cellular Localisation Nucleus
Cytoplasm
Predominantly Nuclear But Also Cytoplasmic
Found In Nuclear Body-Like Structures (Dots) And In A Filamentous Vimentin-Like Pattern
Associated With Tubular Structures
Alternative Antibody Names Anti-Autoimmune Regulator antibody
Anti-Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein antibody
Anti-Apeced Protein antibody
Anti-AIRE antibody
Anti-APECED antibody

Information sourced from Uniprot.org

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