• Western blot analysis of lysates from HeLa cells, treated with Hu 2nM 24h, using AIRE Antibody. The lane on the right is blocked with the synthesized peptide.

Anti-AIRE antibody (126-175 aa) (STJ91519)

SKU:
STJ91519

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Autoimmune regulator (126-175 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
ELISA 1:5000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AIRE
Gene ID: 326
Uniprot ID: AIRE_HUMAN
Immunogen Region: 126-175 aa
Specificity: AIRE-1 Polyclonal Antibody detects endogenous levels of AIRE-1 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human AIRE at the amino acid range 126-175
Post Translational Modifications Phosphorylated. Phosphorylation could trigger oligomerization.
Function Transcription factor playing an essential role to promote self-tolerance in the thymus by regulating the expression of a wide array of self-antigens that have the commonality of being tissue-restricted in their expression pattern in the periphery, called tissue restricted antigens (TRA). Binds to G-doublets in an A/T-rich environment.the preferred motif is a tandem repeat of 5'-ATTGGTTA-3' combined with a 5'-TTATTA-3' box. Binds to nucleosomes. Binds to chromatin and interacts selectively with histone H3 that is not methylated at 'Lys-4', not phosphorylated at 'Thr-3' and not methylated at 'Arg-2'. Functions as a sensor of histone H3 modifications that are important for the epigenetic regulation of gene expression. Mainly expressed by medullary thymic epithelial cells (mTECs), induces the expression of thousands of tissue-restricted proteins, which are presented on major histocompatibility complex class I (MHC-I) and MHC-II molecules to developing T-cells percolating through the thymic medulla. Also induces self-tolerance through other mechanisms such as the regulation of the mTEC differentiation program. Controls the medullary accumulation of thymic dendritic cells and the development of regulatory T-cell through the regulation of XCL1 expression. Regulates the production of CCR4 and CCR7 ligands in medullary thymic epithelial cells and alters the coordinated maturation and migration of thymocytes. In thimic B-cells, allows the presentation of licensing-dependent endogenous self-anitgen for negative selection. In secondary lymphoid organs, induces functional inactivation of CD4(+) T-cells. Expressed by a distinct bone marrow-derived population, induces self-tolerance through a mechanism that does not require regulatory T-cells and is resitant to innate inflammatory stimuli.
Protein Name Autoimmune Regulator
Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein
Apeced Protein
Cellular Localisation Nucleus
Cytoplasm
Predominantly Nuclear But Also Cytoplasmic
Found In Nuclear Body-Like Structures (Dots) And In A Filamentous Vimentin-Like Pattern
Associated With Tubular Structures
Alternative Antibody Names Anti-Autoimmune Regulator antibody
Anti-Autoimmune Polyendocrinopathy Candidiasis Ectodermal Dystrophy Protein antibody
Anti-Apeced Protein antibody
Anti-AIRE antibody
Anti-APECED antibody

Information sourced from Uniprot.org

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