• Biotinylated STJ70166 (0. 1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-AIF1/IBA1, biotinylated-Isoform 1/3 antibody (C-Term) {Biotin} (STJ73271)

SKU:
STJ73271-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-AIF1/IBA1, biotinylated-Isoform 1/3 (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: AIF1
Gene ID: 199
Uniprot ID: AIF1_HUMAN
Immunogen Region: C-Term
Accession Number: NP_116573.1; NP_004838.1; NP_001614.3
Specificity: This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).
Immunogen Sequence: TGPPAKKAISELP.
Post Translational Modifications Phosphorylated on serine residues.
Function Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation.
Protein Name Allograft Inflammatory Factor 1
Aif-1
Ionized Calcium-Binding Adapter Molecule 1
Protein G1
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Ruffle Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Phagocytic Cup
Associated With The Actin Cytoskeleton At Membrane Ruffles And At Sites Of Phagocytosis
Alternative Antibody Names Anti-Allograft Inflammatory Factor 1 antibody
Anti-Aif-1 antibody
Anti-Ionized Calcium-Binding Adapter Molecule 1 antibody
Anti-Protein G1 antibody
Anti-AIF1 antibody
Anti-G1 antibody
Anti-IBA1 antibody

Information sourced from Uniprot.org

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