• Immunofluorescence analysis of U-2 OS cells using AGR2 antibody (STJ29144) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using AGR2 antibody (STJ29144) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded Mouse lung using AGR2 Rabbit polyclonal antibody (STJ29144) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded Human lung cancer using AGR2 Rabbit polyclonal antibody (STJ29144) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using AGR2 antibody (STJ29144) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-AGR2 antibody (21-175) (STJ29144)

SKU:
STJ29144

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-AGR2 (21-175) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: AGR2
Gene ID: 10551
Uniprot ID: AGR2_HUMAN
Immunogen Region: 21-175
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-175 of human AGR2 (NP_006399.1).
Immunogen Sequence: RDTTVKPGAKKDTKDSRPKL PQTLSRGWGDQLIWTQTYEE ALYKSKTSNKPLMIIHHLDE CPHSQALKKVFAENKEIQKL AEQFVLLNLVYETTDKHLSP DGQYVPRIMFVDPSLTVRAD ITGRYSNRLYAYEPADTALL LDNMKKALKLLKTEL
Tissue Specificity Expressed strongly in trachea, lung, stomach, colon, prostate and small intestine. Expressed weakly in pituitary gland, salivary gland, mammary gland, bladder, appendix, ovary, fetal lung, uterus, pancreas, kidney, fetal kidney, testis, placenta, thyroid gland and in estrogen receptor (ER)-positive breast cancer cell lines.
Function Required for MUC2 post-transcriptional synthesis and secretion. May play a role in the production of mucus by intestinal cells. Proto-oncogene that may play a role in cell migration, cell differentiation and cell growth. Promotes cell adhesion.
Protein Name Anterior Gradient Protein 2 Homolog
Ag-2
Hag-2
Hpc8
Secreted Cement Gland Protein Xag-2 Homolog
Cellular Localisation Secreted
Endoplasmic Reticulum
Alternative Antibody Names Anti-Anterior Gradient Protein 2 Homolog antibody
Anti-Ag-2 antibody
Anti-Hag-2 antibody
Anti-Hpc8 antibody
Anti-Secreted Cement Gland Protein Xag-2 Homolog antibody
Anti-AGR2 antibody
Anti-AG2 antibody
Anti-UNQ515 antibody
Anti-PRO1030 antibody

Information sourced from Uniprot.org

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