Anti-AGO4 antibody (STJA0004797)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthetic peptide of human AGO4
General Information
| Short Description | Rabbit polyclonal anti-Protein Argonaute-4 for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.4 mg/mL |
| Purification | Affinity purification |
| Dilution Range | WB 1:500-1:2000 |
| Formulation | PBS with 0.05% sodium azide and 50% glycerol, PH7.4 |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | AGO4 |
| Gene ID | 192670 |
| Uniprot ID | AGO4_HUMAN |
| Immunogen | Synthetic peptide of human AGO4 |
Additional Info
| Post Translational Modifications | Ubiquitinated on surface-exposed lysines by a SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 during target-directed microRNA degradation (TDMD), a process that mediates degradation of microRNAs (miRNAs). Ubiquitination by the SCF-like E3 ubiquitin-protein ligase complex containing ZSWIM8 leads to its subsequent degradation, thereby exposing miRNAs for degradation. ZSWIM8 recognizes and binds AGO4 when it is engaged with a TDMD target. |
| Function | Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV). |
| Protein Name | Protein Argonaute-4Argonaute4Hago4Argonaute Risc Catalytic Component 4Eukaryotic Translation Initiation Factor 2c 4Eif-2c 4Eif2c 4 |
| Database Links | Reactome: R-HSA-1912408Reactome: R-HSA-203927Reactome: R-HSA-2559580Reactome: R-HSA-2559585Reactome: R-HSA-4086398Reactome: R-HSA-426486Reactome: R-HSA-426496Reactome: R-HSA-5628897Reactome: R-HSA-5687128Reactome: R-HSA-8853884Reactome: R-HSA-8934593Reactome: R-HSA-8936459Reactome: R-HSA-8943723Reactome: R-HSA-8948700Reactome: R-HSA-8986944Reactome: R-HSA-9018519Reactome: R-HSA-9022692Reactome: R-HSA-9029569Reactome: R-HSA-9725371Reactome: R-HSA-9759811Reactome: R-HSA-9768778Reactome: R-HSA-9824594Reactome: R-HSA-9839394 |
| Cellular Localisation | CytoplasmP-Body |
| Alternative Antibody Names | Anti-Protein Argonaute-4 antibodyAnti-Argonaute4 antibodyAnti-Hago4 antibodyAnti-Argonaute Risc Catalytic Component 4 antibodyAnti-Eukaryotic Translation Initiation Factor 2c 4 antibodyAnti-Eif-2c 4 antibodyAnti-Eif2c 4 antibodyAnti-AGO4 antibodyAnti-EIF2C4 antibodyAnti-KIAA1567 antibody |
Information sourced from Uniprot.org