Anti-AFF4 antibody (30-110 aa) (STJ190525)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 30-110
General Information
| Short Description | Rabbit polyclonal anti-AF4/FMR2 family member 4 (30-110 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | AFF4 |
| Gene ID | 27125 |
| Uniprot ID | AFF4_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 30-110 |
| Immunogen Region | 30-110 aa |
| Specificity | AFF4 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Dephosphorylated at Ser-549 by the PNUTS-PP1 complex, promoting RNA polymerase II transcription pause-release. |
| Function | Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through direct interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression. |
| Protein Name | Af4/Fmr2 Family Member 4All1-Fused Gene From Chromosome 5q31 ProteinProtein Af-5q31Major Cdk9 Elongation Factor-Associated Protein |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-674695Reactome: R-HSA-75955 |
| Cellular Localisation | NucleusChromosomeAssociates To Transcriptionally Active Chromatin But Not At Snrna Genes |
| Alternative Antibody Names | Anti-Af4/Fmr2 Family Member 4 antibodyAnti-All1-Fused Gene From Chromosome 5q31 Protein antibodyAnti-Protein Af-5q31 antibodyAnti-Major Cdk9 Elongation Factor-Associated Protein antibodyAnti-AFF4 antibodyAnti-AF5Q31 antibodyAnti-MCEF antibodyAnti-HSPC092 antibody |
Information sourced from Uniprot.org