| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-AFAP1 (461-730) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:2000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
AFAP1 |
| Gene ID: |
60312 |
| Uniprot ID: |
AFAP1_HUMAN |
| Immunogen Region: |
461-730 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 461-730 of human AFAP1 (NP_940997.1). |
| Immunogen Sequence: |
SVIQTAKQTFCFMNRRVISA NPYLGGTSNGYAHPSGTALH YDDVPCINGSLKGKKPPVAS NGVTGKGKTLSSQPKKADPA AVVKRTGSNAAQYKYGKNRV EADAKRLQTKEEELLKRKEA LRNRLAQLRKERKDLRAAIE VNAGRKPQAILEEKLKQLEE ECRQKEAERVSLELELTEVK ESLKKALAGGVTLGLAIEPK SGTSSPQSPVFRHRTLENSP ISSCDTSDTEGPVPVNSAA |
| Tissue Specificity | Low expression in normal breast epithelial cell line MCF-10A and in tumorigenic breast cancer cell lines MCF-7, T-47D and ZR-75-1. Highly expressed in the invasive breast cancer cell lines MDA-MB-231 and MDA-MB-435. Overexpressed in prostate carcinoma. |
| Post Translational Modifications | Phosphorylated on tyrosine residues by SRC. |
| Function | Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells. |
| Protein Name | Actin Filament-Associated Protein 1110 Kda Actin Filament-Associated ProteinAfap-110 |
| Cellular Localisation | CytoplasmCytoskeletonStress Fiber |
| Alternative Antibody Names | Anti-Actin Filament-Associated Protein 1 antibodyAnti-110 Kda Actin Filament-Associated Protein antibodyAnti-Afap-110 antibodyAnti-AFAP1 antibodyAnti-AFAP antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
