Anti-ADIPOQ antibody (C-Term) [M577] (STJA0005248)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenClone (M577) was generated from a recombinant protein that included amino acid residues within the C-terminal region of mouse adiponectin. This sequence has high homology with similar regions in rat and human adiponectin.
General Information
| Short Description | Mouse monoclonal antibody anti-Adiponectin (C-Term) is suitable for use in Western Blot research applications. |
| Applications | WB |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | M577 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | Protein G Purified |
| Dilution Range | WB 1:1000 |
| Formulation | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Adipoq |
| Gene ID | 11450 |
| Uniprot ID | ADIPO_MOUSE |
| Immunogen | Clone (M577) was generated from a recombinant protein that included amino acid residues within the C-terminal region of mouse adiponectin. This sequence has high homology with similar regions in rat and human adiponectin. |
| Immunogen Region | C-Term |
| Specificity | This antibody detects a 30 kDa* protein on SDS-PAGE immunoblots of human and mouse serum, as well as detects full length human and mouse recombinant proteins. |
Additional Info
| Tissue Specificity | Synthesized exclusively by adipocytes and secreted into plasma. |
| Post Translational Modifications | HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagen-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated O-linked glycans on hydroxylysine residues consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. O-linked glycosylation in the N-terminal is disialylated with the structure Neu5Acalpha2->8Neu5Acalpha2->3Gal. Sialylated by alpha 2,8-sialyltransferase III. Succination of Cys-39 by the Krebs cycle intermediate fumarate, which leads to S-(2-succinyl)cysteine residues, inhibits polymerization and secretion of adiponectin. Adiponectin is a major target for succination in both adipocytes and adipose tissue of diabetic mice. It was proposed that succination of proteins is a biomarker of mitochondrial stress and accumulation of Krebs cycle intermediates in adipose tissue in diabetes and that succination of adiponectin may contribute to the decrease in plasma adiponectin in diabetes. |
| Function | Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. |
| Protein Name | Adiponectin30 Kda Adipocyte Complement-Related ProteinAdipocyte Complement-Related 30 Kda ProteinAcrp30Adipocyte - C1q And Collagen Domain-Containing ProteinAdipocyte-Specific Protein Adipoq |
| Database Links | Reactome: R-MMU-163680 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Adiponectin antibodyAnti-30 Kda Adipocyte Complement-Related Protein antibodyAnti-Adipocyte Complement-Related 30 Kda Protein antibodyAnti-Acrp30 antibodyAnti-Adipocyte - C1q And Collagen Domain-Containing Protein antibodyAnti-Adipocyte-Specific Protein Adipoq antibodyAnti-Adipoq antibodyAnti-Acdc antibodyAnti-Acrp30 antibodyAnti-Apm1 antibody |
Information sourced from Uniprot.org