• Western blot analysis of extracts of various cell lines, using ADI1 antibody (STJ116261) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunohistochemistry of paraffin-embedded human breast cancer using ADI1 rabbit polyclonal antibody (STJ116261) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse kidney using ADI1 rabbit polyclonal antibody (STJ116261) at dilution of 1:200 (40x lens).
  • Immunohistochemistry of paraffin-embedded rat kidney using ADI1 rabbit polyclonal antibody (STJ116261) at dilution of 1:200 (40x lens).

Anti-ADI1 antibody (1-179) (STJ116261)

SKU:
STJ116261

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ADI1 (1-179) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ADI1
Gene ID: 55256
Uniprot ID: MTND_HUMAN
Immunogen Region: 1-179
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human ADI1 (NP_060739.2).
Immunogen Sequence: MVQAWYMDDAPGDPRQPHRP DPGRPVGLEQLRRLGVLYWK LDADKYENDPELEKIRRERN YSWMDIITICKDKLPNYEEK IKMFYEEHLHLDDEIRYILD GSGYFDVRDKEDQWIRIFME KGDMVTLPAGIYHRFTVDEK NYTKAMRLFVGEPVWTAYNR PADHFEARGQYVKFLAQTA
Tissue Specificity Detected in heart, colon, lung, stomach, brain, spleen, liver, skeletal muscle and kidney.
Function Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway. Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line.
Protein Name Acireductone Dioxygenase
Acireductone Dioxygenase
Fe(2+-Requiring
Ard'
Fe-Ard
Acireductone Dioxygenase
Ni(2+-Requiring
Ard
Ni-Ard
Membrane-Type 1 Matrix Metalloproteinase Cytoplasmic Tail-Binding Protein 1
Mtcbp-1
Submergence-Induced Protein-Like Factor
Sip-L
Database Links Reactome: R-HSA-1237112
Cellular Localisation Cytoplasm
Nucleus
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Localizes To The Plasma Membrane When Complexed To Mmp14
Alternative Antibody Names Anti-Acireductone Dioxygenase antibody
Anti-Acireductone Dioxygenase antibody
Anti-Fe(2+-Requiring antibody
Anti-Ard' antibody
Anti-Fe-Ard antibody
Anti-Acireductone Dioxygenase antibody
Anti-Ni(2+-Requiring antibody
Anti-Ard antibody
Anti-Ni-Ard antibody
Anti-Membrane-Type 1 Matrix Metalloproteinase Cytoplasmic Tail-Binding Protein 1 antibody
Anti-Mtcbp-1 antibody
Anti-Submergence-Induced Protein-Like Factor antibody
Anti-Sip-L antibody
Anti-ADI1 antibody
Anti-MTCBP1 antibody
Anti-HMFT1638 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance