| Host: |
Rabbit |
| Applications: |
WB/IHC |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-ADI1 (1-179) is suitable for use in Western Blot and Immunohistochemistry research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ADI1 |
| Gene ID: |
55256 |
| Uniprot ID: |
MTND_HUMAN |
| Immunogen Region: |
1-179 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human ADI1 (NP_060739.2). |
| Immunogen Sequence: |
MVQAWYMDDAPGDPRQPHRP DPGRPVGLEQLRRLGVLYWK LDADKYENDPELEKIRRERN YSWMDIITICKDKLPNYEEK IKMFYEEHLHLDDEIRYILD GSGYFDVRDKEDQWIRIFME KGDMVTLPAGIYHRFTVDEK NYTKAMRLFVGEPVWTAYNR PADHFEARGQYVKFLAQTA |
| Tissue Specificity | Detected in heart, colon, lung, stomach, brain, spleen, liver, skeletal muscle and kidney. |
| Function | Catalyzes 2 different reactions between oxygen and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4-methylthiobutyrate (KMTB), the alpha-ketoacid precursor of methionine in the methionine recycle pathway. Ni-containing acireductone dioxygenase (Ni-ARD) produces methylthiopropionate, carbon monoxide and formate, and does not lie on the methionine recycle pathway. Also down-regulates cell migration mediated by MMP14. Necessary for hepatitis C virus replication in an otherwise non-permissive cell line. |
| Protein Name | Acireductone DioxygenaseAcireductone DioxygenaseFe(2+-RequiringArd'Fe-ArdAcireductone DioxygenaseNi(2+-RequiringArdNi-ArdMembrane-Type 1 Matrix Metalloproteinase Cytoplasmic Tail-Binding Protein 1Mtcbp-1Submergence-Induced Protein-Like FactorSip-L |
| Database Links | Reactome: R-HSA-1237112 |
| Cellular Localisation | CytoplasmNucleusCell MembranePeripheral Membrane ProteinCytoplasmic SideLocalizes To The Plasma Membrane When Complexed To Mmp14 |
| Alternative Antibody Names | Anti-Acireductone Dioxygenase antibodyAnti-Acireductone Dioxygenase antibodyAnti-Fe(2+-Requiring antibodyAnti-Ard' antibodyAnti-Fe-Ard antibodyAnti-Acireductone Dioxygenase antibodyAnti-Ni(2+-Requiring antibodyAnti-Ard antibodyAnti-Ni-Ard antibodyAnti-Membrane-Type 1 Matrix Metalloproteinase Cytoplasmic Tail-Binding Protein 1 antibodyAnti-Mtcbp-1 antibodyAnti-Submergence-Induced Protein-Like Factor antibodyAnti-Sip-L antibodyAnti-ADI1 antibodyAnti-MTCBP1 antibodyAnti-HMFT1638 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and UBE2C knockout (KO) HeLa cells, using [KO Validated] UBE2C Rabbit polyclonal antibody (STJ11100048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89120/357572/STJ11100048_1__15924.1713121701.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and UBE2C knockout (KO) HeLa cells, using [KO Validated] UBE2C Rabbit polyclonal antibody (STJ11100048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
