Anti-ADHFE1 antibody (41-255) (STJ11105629)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal Hydroxyacid-Oxoacid Transhydrogenase-Mitochondrial (41-255) antibody for use in WB and ELISA in mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ADHFE1 |
| Gene ID | 137872 |
| Uniprot ID | HOT_HUMAN |
| Immunogen Region | 41-255 |
| Immunogen Sequence | KTTDYAFEMAVSNIRYGAAV TKEVGMDLKNMGAKNVCLMT DKNLSKLPPVQVAMDSLVKN GIPFTVYDNVRVEPTDSSFM EAIEFAQKGAFDAYVAVGGG STMDTCKAANLYASSPHSDF LDYVSAPIGKGKPVSVPLKP LIAVPTTSGTGSETTGVAIF DYEHLKVKIGITSRAIKPTL GLIDPLHTLHMPARVVANSG FDVLCHALESYTTLP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 41-255 of human ADHFE1 (NP_653251.2). |
Additional Info
| Tissue Specificity | Only expressed in adult liver. |
| Function | Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). D,L-3-hydroxyisobutyrate and L-3-hydroxybutyrate (L-3-OHB) are also substrates for HOT with 10-fold lower activities. |
| Protein Name | Hydroxyacid-Oxoacid Transhydrogenase - MitochondrialHotAlcohol Dehydrogenase Iron-Containing Protein 1Adhfe1Fe-Containing Alcohol Dehydrogenase |
| Database Links | Reactome: R-HSA-880009 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Hydroxyacid-Oxoacid Transhydrogenase - Mitochondrial antibodyAnti-Hot antibodyAnti-Alcohol Dehydrogenase Iron-Containing Protein 1 antibodyAnti-Adhfe1 antibodyAnti-Fe-Containing Alcohol Dehydrogenase antibodyAnti-ADHFE1 antibodyAnti-HMFT2263 antibody |
Information sourced from Uniprot.org