Anti-ADGRL3 antibody (STJ192663)

SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from part of the human protein
STJ192663
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General Information

Short DescriptionRabbit polyclonal anti-Adhesion G protein-coupled receptor L3 for use in WB and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
Concentration1 mg/mL
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Dilution RangeWB 1:500-2000
ELISA 1:5000-20000
FormulationLiquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Storage InstructionStore at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolADGRL3
Gene ID23284
Uniprot IDAGRL3_HUMAN
ImmunogenSynthesized peptide derived from part of the human protein
SpecificityLPHN3 Polyclonal Antibody detects endogenous levels of protein.

Additional Info

Function Orphan adhesion G-protein coupled receptor (aGPCR), which mediates synapse specificity. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. ADGRL3 is coupled with different classes of G alpha proteins, such as G(12)/G(13), G(s), G(i) or G(q), depending on the context. Coupling to G(12)/G(13) G proteins, which mediates the activation Rho small GTPases is the most efficient. Following G-protein coupled receptor activation, associates with cell adhesion molecules that are expressed at the surface of adjacent cells to direct synapse specificity. Specifically mediates the establishment of Schaffer-collateral synapses formed by CA3-region axons on CA1-region pyramidal neurons in the hippocampus. Localizes to postsynaptic spines in excitatory synapses in the S.oriens and S.radiatum and interacts with presynaptic cell adhesion molecules FLRT3 and TENM2, promoting synapse formation. Plays a role in the development of glutamatergic synapses in the cortex. Important in determining the connectivity rates between the principal neurons in the cortex. Isoform 1: Orphan adhesion G-protein coupled receptor (aGPCR), which mediates synapse specificity. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase. Isoform 1 is specifically coupled to G(s) G proteins and mediates activation of adenylate cyclase activity. Following G-protein coupled receptor activation, undergoes liquid-liquid phase transition, associates with (1) cell adhesion molecules that are expressed at the surface of adjacent cells, as well as (2) PDZ-containing proteins, such as SHANK3 and DLG4, in the cytoplasm to direct synapse formation.
Protein Name Adhesion G Protein-Coupled Receptor L3
Calcium-Independent Alpha-Latrotoxin Receptor 3
Cirl-3
Latrophilin-3
Lectomedin-3
Database Links
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Postsynaptic Cell Membrane
Cell Projection
Axon
Cell Junction
Alternative Antibody Names Anti-Adhesion G Protein-Coupled Receptor L3 antibody
Anti-Calcium-Independent Alpha-Latrotoxin Receptor 3 antibody
Anti-Cirl-3 antibody
Anti-Latrophilin-3 antibody
Anti-Lectomedin-3 antibody
Anti-ADGRL3 antibody
Anti-KIAA0768 antibody
Anti-LEC3 antibody
Anti-LPHN3 antibody

Information sourced from Uniprot.org

Citations

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