Anti-Adenosine triphosphate-ase antibody (aa: 147-161) (STJA0040840)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeMixed
ImmunogenA synthetic peptide (C-ELHRQEEAKQVLRYY) as part of mouse ATP13A2 protein (aa: 147-161) conjugated to KLH
General Information
| Short Description | Rabbit polyclonal anti-Adenosine triphosphate-ase (aa: 147-161) for use in IHC-F in Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC-F |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | Mixed |
| Conjugation | Unconjugated |
| Purification | Whole serum |
| Dilution Range | IHC 1:500-1:2000 |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at-20°C for a higher stability, and at 2-8°C with an appropriate antibacterial |
Target Information
| Gene Symbol | Atp13a2 |
| Gene ID | 74772 |
| Uniprot ID | AT132_MOUSE |
| Immunogen | A synthetic peptide (C-ELHRQEEAKQVLRYY) as part of mouse ATP13A2 protein (aa: 147-161) conjugated to KLH |
| Immunogen Region | aa: 147-161 |
| Immunogen Sequence | Mouse |
| Specificity | Immunohistochemistry shows specific staining for ATPase. This antiserum is known to react with rat ATPase. Other species have not yet tested. |
Additional Info
| Post Translational Modifications | Autophosphorylated. Accumulates in an inactive autophosphorylated state and autophosphorylation is stimulated by phosphatidic acid and phosphatidylinositol 3,5-bisphosphate but not by Mn(2+) or Zn(2+). The presence of spermine results in a dose-dependent reduction in autophosphorylation. |
| Function | ATPase which acts as a lysosomal polyamine exporter with high affinity for spermine. Also stimulates cellular uptake of polyamines and protects against polyamine toxicity. Plays a role in intracellular cation homeostasis and the maintenance of neuronal integrity. Contributes to cellular zinc homeostasis. Confers cellular protection against Mn(2+) and Zn(2+) toxicity and mitochondrial stress. Required for proper lysosomal and mitochondrial maintenance. Regulates the autophagy-lysosome pathway through the control of SYT11 expression at both transcriptional and post-translational levels. Facilitates recruitment of deacetylase HDAC6 to lysosomes to deacetylate CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy. Promotes secretion of exosomes as well as secretion of SCNA via exosomes. Plays a role in lipid homeostasis. |
| Protein Name | Polyamine-Transporting Atpase 13a2 |
| Database Links | Reactome: R-MMU-936837 |
| Cellular Localisation | Lysosome MembraneMulti-Pass Membrane ProteinLate Endosome MembraneEndosomeMultivesicular Body MembraneCytoplasmic VesicleAutophagosome Membrane |
| Alternative Antibody Names | Anti-Polyamine-Transporting Atpase 13a2 antibodyAnti-Atp13a2 antibody |
Information sourced from Uniprot.org