• STJ70149 staining (0. 2µg/ml) of Human HeLa lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.

Anti-ADAM17/TACE antibody (C-Term) (STJ70149)

SKU:
STJ70149-100

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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ADAM17/TACE (C-Term) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-Recommended for use at 0.1-1µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ADAM17
Gene ID: 6868
Uniprot ID: ADA17_HUMAN
Immunogen Region: C-Term
Accession Number: NP_003174.3
Specificity: This antibody is expected to recognise isoform 1 (represented by NP_003174.3) and not isoform 2 (represented by NP_068604).
Immunogen Sequence: LQRQNRVDSKETEC
Post Translational Modifications The precursor is cleaved by a furin endopeptidase. Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. Phosphorylation at Thr-735 by MAPK14 is required for ADAM17-mediated ectodomain shedding.
Function Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Acts as an activator of Notch pathway by mediating cleavage of Notch, generating the membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). Plays a role in the proteolytic processing of ACE2. Plays a role in hemostasis through shedding of GP1BA, the platelet glycoprotein Ib alpha chain. Mediates the proteolytic cleavage of LAG3, leading to release the secreted form of LAG3. Mediates the proteolytic cleavage of IL6R, leading to the release of secreted form of IL6R. Mediates the proteolytic cleavage and shedding of FCGR3A upon NK cell stimulation, a mechanism that allows for increased NK cell motility and detachment from opsonized target cells.
Protein Name Disintegrin And Metalloproteinase Domain-Containing Protein 17
Adam 17
Snake Venom-Like Protease
Tnf-Alpha Convertase
Tnf-Alpha-Converting Enzyme
Cd Antigen Cd156b
Database Links Reactome: R-HSA-1251985
Reactome: R-HSA-1442490
Reactome: R-HSA-177929
Reactome: R-HSA-193692
Reactome: R-HSA-2122948
Reactome: R-HSA-2644606
Reactome: R-HSA-2660826
Reactome: R-HSA-2691232
Reactome: R-HSA-2894862
Reactome: R-HSA-5362798
Reactome: R-HSA-75893
Reactome: R-HSA-9662834
Reactome: R-HSA-982772
Cellular Localisation Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Disintegrin And Metalloproteinase Domain-Containing Protein 17 antibody
Anti-Adam 17 antibody
Anti-Snake Venom-Like Protease antibody
Anti-Tnf-Alpha Convertase antibody
Anti-Tnf-Alpha-Converting Enzyme antibody
Anti-Cd Antigen Cd156b antibody
Anti-ADAM17 antibody
Anti-CSVP antibody
Anti-TACE antibody

Information sourced from Uniprot.org

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