• Confocal imaging of U-2 OS cells using Arp3 Rabbit monoclonal antibody (STJ11102091, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
  • Immunofluorescence analysis of C6 cells using Arp3 Rabbit monoclonal antibody (STJ11102091) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Arp3 in paraffin-embedded rat brain tissue using Arp3 Rabbit monoclonal antibody (STJ11102091) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Arp3 in paraffin-embedded mouse spleen tissue using Arp3 Rabbit monoclonal antibody (STJ11102091) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Arp3 in paraffin-embedded human tonsil tissue using Arp3 Rabbit monoclonal antibody (STJ11102091) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of Arp3 in paraffin-embedded human colon carcinoma tissue using Arp3 Rabbit monoclonal antibody (STJ11102091) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of extracts of various cell lines, using Arp3 Rabbit monoclonal antibody (STJ11102091) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-ACTR3 antibody (319-418) [S1MR] (STJ11102091)

SKU:
STJ11102091

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Arp3 (319-418) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S1MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTR3
Gene ID: 10096
Uniprot ID: ARP3_HUMAN
Immunogen Region: 319-418
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 319-418 of human Arp3 (P61158).
Immunogen Sequence: IVLSGGSTMFRDFGRRLQRD LKRTVDARLKLSEELSGGRL KPKPIDVQVITHHMQRYAVW FGGSMLASTPEFYQVCHTKK DYEEIGPSICRHNPVFGVMS
Function ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. Seems to contact the pointed end of the daughter actin filament. In podocytes, required for the formation of lamellipodia downstream of AVIL and PLCE1 regulation. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs). Plays a role in ciliogenesis.
Protein Name Actin-Related Protein 3
Actin-Like Protein 3
Database Links Reactome: R-HSA-2029482
Reactome: R-HSA-3928662
Reactome: R-HSA-5663213
Reactome: R-HSA-8856828
Reactome: R-HSA-9664422
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Projection
Nucleus
In Pre-Apoptotic Cells
Colocalizes With Mefv In Large Specks (Pyroptosomes)
Alternative Antibody Names Anti-Actin-Related Protein 3 antibody
Anti-Actin-Like Protein 3 antibody
Anti-ACTR3 antibody
Anti-ARP3 antibody

Information sourced from Uniprot.org

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