• Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g Alpha-Actinin-4 antibody (STJ11101857). Western blot was performed from the immunoprecipitate using Alpha-Actinin-4 antibody (STJ11101857) at a dilution of 1:1000.
  • Confocal imaging of U-2 OS cells using  Alpha-Actinin-4 Rabbit monoclonal antibody (STJ11101857, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Western blot analysis of various lysates using Alpha-Actinin-4 Rabbit monoclonal antibody (STJ11101857) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ACTN4 antibody (1-100) [S7MR] (STJ11101857)

SKU:
STJ11101857

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-alpha Actinin 4 (1-100) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTN4
Gene ID: 81
Uniprot ID: ACTN4_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Alpha-Actinin-4 (O43707).
Immunogen Sequence: MVDYHAANQSYQYGPSSAGN GAGGGGSMGDYMAQEDDWDR DLLLDPAWEKQQRKTFTAWC NSHLRKAGTQIENIDEDFRD GLKLMLLLEVISGERLPKPE
Tissue Specificity Widely expressed.
Function F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein (Probable). Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions. May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA.
Protein Name Alpha-Actinin-4
Non-Muscle Alpha-Actinin 4
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-373753
Cellular Localisation Nucleus
Cytoplasm
Cell Junction
Cytoskeleton
Stress Fiber
Perinuclear Region
Localized In Cytoplasmic Mrnp Granules Containing Untranslated Mrnas
Expressed In The Perinuclear Rim And Manchette Structure In Early Elongating Spermatids During Spermiogenesis
Nuclear Translocation Can Be Induced By The Pi3 Kinase Inhibitor Wortmannin Or By Cytochalasin D
Exclusively Localized In The Nucleus In A Limited Number Of Cell Lines (Breast Cancer Cell Line Mcf-7
Oral Floor Cancer Imc-2
And Bladder Cancer Ku-7)
Alternative Antibody Names Anti-Alpha-Actinin-4 antibody
Anti-Non-Muscle Alpha-Actinin 4 antibody
Anti-ACTN4 antibody

Information sourced from Uniprot.org

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