Anti-Actin antibody [5J11] (STJA0040767)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1
ImmunogenActin prepared from bovine brain.
General Information
| Short Description | Mouse monoclonal anti-Actin for use in FC, ICC and WB in Bovine, Horse, Human, Pig and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | FC/ICC/WB |
| Host | Mouse |
| Reactivity | Bovine/Horse/Human/Pig/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 5J11 |
| Isotype | IgG1 |
| Conjugation | Unconjugated |
| Purification | IgG |
| Dilution Range | WB 1:5000-1:10000ICC 1:500-1:1000 |
| Formulation | Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide |
| Storage Instruction | Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at-20°C for |
Target Information
| Immunogen | Actin prepared from bovine brain. |
| Immunogen Sequence | Bovine |
| Specificity | The antibody reacts with a 42 kDa band by Western blot on a crude extract from HeLa cells. It has also been used successfully for immunocytochemistry. It reacts across a broad range of mammalian species. |
Additional Info
| Background | Our Anti-Actin mouse monoclonal primary antibody detects bovine, horse, human, pig, and rat Actin, and is igg. It is validated for use in FC, ICC, WB. |
Information sourced from Uniprot.org