• Western blot analysis of 1) Hela, 2) Mouse Brain tissue, 3) Rat Brain tissue, diluted at 1:20000.
  • 1) Input: Mouse Brain Tissue Lysate , 2) IP product: IP dilute 1: 200
  • Immunofluorescence analysis of Rat-liver tissue. 1, Alpha skeletal muscle actin monoclonal antibody (4B11) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Mouse-liver tissue. 1, Alpha skeletal muscle actin monoclonal antibody (4B11) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunofluorescence analysis of Human-liver-cancer tissue. 1, Alpha skeletal muscle actin monoclonal antibody (4B11) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
  • Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, Alpha skeletal muscle actin monoclonal antibody (4B11) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, Alpha skeletal muscle actin monoclonal antibody (4B11) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-ACTA1 antibody [4B11] (STJ97051)

SKU:
STJ97051

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Actin, alpha skeletal muscle is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: 4B11
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS pH7.4, 0.5% BSA, 0.02% Sodium Azide and 50% Glycerol.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: WB 1:500-10000
IP 1:200
IF 1:200
IHC 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTA1
Gene ID: 58
Uniprot ID: ACTS_HUMAN
Specificity: The antibody detects endogenous Alpha Skeletal Muscle Actin protein.
Immunogen: Synthetic Peptide of Alpha skeletal muscle actin
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Protein Name Actin - Alpha Skeletal Muscle
Alpha-Actin-1 Cleaved Into - Actin - Alpha Skeletal Muscle - Intermediate Form
Database Links Reactome: R-HSA-390522
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Actin - Alpha Skeletal Muscle antibody
Anti-Alpha-Actin-1 Cleaved Into - Actin - Alpha Skeletal Muscle - Intermediate Form antibody
Anti-ACTA1 antibody
Anti-ACTA antibody

Information sourced from Uniprot.org

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