• Western blot of rat hippocampal lysate showing specific immunolabeling of the ~42 kDa actin protein.
  • Immunofluorescence staining of human blood-brain barrier endothelial cells, HMEC 3, showing specific labeling of actin (STJA0003575, red, 1:200) The cell nuclei are stained in blue with DAPI. Photo credit of Yancy Ferrer-Acosta/Henrique Martins, Universidad Central Del Caribe, PR.

Anti-ACTA1 antibody [C4] (STJA0003575)

SKU:
STJA0003575-100

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Host: Mouse
Applications: WB/IHC/ICC
Reactivity: All
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Actin is suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: C4
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Ascites fluid + 0.01 % Sodium Azide.
Purification: Ascites fluid.
Dilution Range: WB 1:5000
IHC 1:500
ICC 1:200-1:500
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: Preparation of chicken gizzard actin.
Background Actin is the most abundant protein found in virtually all eukaryotic cells. It is also one of the most highly-conserved proteins, differing by no more than 20% in species as diverse as algae and humans, making it an excellent choice for use as a loading control. It is the monomeric subunit of microfilaments, one of the three major components of the cytoskeleton, and of thin filaments, which are part of the contractile apparatus in muscle cells. Thus, actin participates in many important cellular functions, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape.

Information sourced from Uniprot.org

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