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Anti-ACSL4 antibody (42-321) (STJ28909)

Immunoprecipitation analysis of 300ug extracts of Hep G2 cells using 3ug ACSL4 Rabbit polyclonal antibody antibody (STJ28909 1:100). Western blot was performed from the immunoprecipitate using ACSL4 Rabbit polyclonal antibody antibody (STJ28909) at a dilition of 1:2000.
Immunofluorescence analysis of NIH/3T3 cells using ACSL4 Rabbit polyclonal antibody (STJ28909) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of lysates from Hep G2 cells using ACSL4 Rabbit polyclonal antibody (STJ28909) at 1:400 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:10s.

Anti-ACSL4 antibody (42-321) (STJ28909)

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STJ28909

£169.50 - £449.50

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General Information
Product Properties
Target Information
Additional Info
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Host:	Rabbit
Applications:	WB/IP/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-ACSL4 (42-321) is suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IP 1:1000-1:2000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	ACSL4
Gene ID:	2182
Uniprot ID:	ACSL4_HUMAN
Immunogen Region:	42-321
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 42-321 of human ACSL4 (NP_075266.1?).
Immunogen Sequence:	MAKRIKAKPTSDKPGSPYRS VTHFDSLAVIDIPGADTLDK LFDHAVSKFGKKDSLGTREI LSEENEMQPNGKVFKKLILG NYKWMNYLEVNRRVNNFGSG LTALGLKPKNTIAIFCETRA EWMIAAQTCFKYNFPLVTLY ATLGKEAVVHGLNESEASYL ITSVELLESKLKTALLDISC VKHIIYVDNKAINKAEYPEG FEIHSMQSVEELGSNPENLG IPPSRPTPSDMAIVMYTSG

Function	Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs. Modulates prostaglandin E2 secretion.
Protein Name	Long-Chain-Fatty-Acid--Coa Ligase 4 Arachidonate--Coa Ligase Long-Chain Acyl-Coa Synthetase 4 Lacs 4
Database Links	Reactome: R-HSA-434313 Reactome: R-HSA-75876
Cellular Localisation	Mitochondrion Outer Membrane Single-Pass Type Iii Membrane Protein Peroxisome Membrane Microsome Membrane Endoplasmic Reticulum Membrane Cell Membrane
Alternative Antibody Names	Anti-Long-Chain-Fatty-Acid--Coa Ligase 4 antibody Anti-Arachidonate--Coa Ligase antibody Anti-Long-Chain Acyl-Coa Synthetase 4 antibody Anti-Lacs 4 antibody Anti-ACSL4 antibody Anti-ACS4 antibody Anti-FACL4 antibody Anti-LACS4 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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