• Western blot analysis of Rat liver, using ACSL4 antibody (STJ11103869) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of HepG2, using ACSL4 antibody (STJ11103869) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-ACSL4 antibody (1-280) (STJ11103869)

SKU:
STJ11103869

£116.50 - £543.50
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Long-Chain-Fatty-Acid--Coa Ligase 4 (1-280) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACSL4
Gene ID: 2182
Uniprot ID: ACSL4_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ACSL4 (NP_004449.1).
Immunogen Sequence: MAKRIKAKPTSDKPGSPYRS VTHFDSLAVIDIPGADTLDK LFDHAVSKFGKKDSLGTREI LSEENEMQPNGKVFKKLILG NYKWMNYLEVNRRVNNFGSG LTALGLKPKNTIAIFCETRA EWMIAAQTCFKYNFPLVTLY ATLGKEAVVHGLNESEASYL ITSVELLESKLKTALLDISC VKHIIYVDNKAINKAEYPEG FEIHSMQSVEELGSNPENLG IPPSRPTPSDMAIVMYTSG
Function Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs. Modulates prostaglandin E2 secretion.
Protein Name Long-Chain-Fatty-Acid--Coa Ligase 4
Arachidonate--Coa Ligase
Long-Chain Acyl-Coa Synthetase 4
Lacs 4
Database Links Reactome: R-HSA-434313
Reactome: R-HSA-75876
Cellular Localisation Mitochondrion Outer Membrane
Single-Pass Type Iii Membrane Protein
Peroxisome Membrane
Microsome Membrane
Endoplasmic Reticulum Membrane
Cell Membrane
Alternative Antibody Names Anti-Long-Chain-Fatty-Acid--Coa Ligase 4 antibody
Anti-Arachidonate--Coa Ligase antibody
Anti-Long-Chain Acyl-Coa Synthetase 4 antibody
Anti-Lacs 4 antibody
Anti-ACSL4 antibody
Anti-ACS4 antibody
Anti-FACL4 antibody
Anti-LACS4 antibody

Information sourced from Uniprot.org

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