• Immunofluorescence analysis of A431 cells using ACOT9 Rabbit polyclonal antibody (STJ117611) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ACOT9 Rabbit polyclonal antibody (STJ117611) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ACOT9 antibody (22-250) (STJ117611)

SKU:
STJ117611

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACOT9 (22-250) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACOT9
Gene ID: 23597
Uniprot ID: ACOT9_HUMAN
Immunogen Region: 22-250
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 22-250 of human ACOT9 (NP_001028755.2).
Immunogen Sequence: LTQGPQNPKKQGIFHIHEVR DKLREIVGASTNWRDHVKAM EERKLLHSFLAKSQDGLPPR RMKDSYIEVLLPLGSEPELR EKYLTVQNTVRFGRILEDLD SLGVLICYMHNKIHSAKMSP LSIVTALVDKIDMCKKSLSP EQDIKFSGHVSWVGKTSMEV KMQMFQLHGDEFCPVLDATF VMVARDSENKGPAFVNPLIP ESPEEEELFRQGELNKGRRI AFSSTSLLK
Function Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs.
Protein Name Acyl-Coenzyme A Thioesterase 9 - Mitochondrial
Acyl-Coa Thioesterase 9
Acyl-Coa Thioester Hydrolase 9
Database Links Reactome: R-HSA-77289
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Acyl-Coenzyme A Thioesterase 9 - Mitochondrial antibody
Anti-Acyl-Coa Thioesterase 9 antibody
Anti-Acyl-Coa Thioester Hydrolase 9 antibody
Anti-ACOT9 antibody
Anti-CGI-16 antibody

Information sourced from Uniprot.org

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