• Immunohistochemistry analysis of ACOT2 in paraffin-embedded mouse kidney using ACOT2 Rabbit polyclonal antibody (STJ116602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of ACOT2 in paraffin-embedded human appendix using ACOT2 Rabbit polyclonal antibody (STJ116602) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using ACOT2 Rabbit polyclonal antibody (STJ116602) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ACOT2 antibody (222-483) (STJ116602)

SKU:
STJ116602

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACOT2 (222-483) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACOT2
Gene ID: 10965
Uniprot ID: ACOT2_HUMAN
Immunogen Region: 222-483
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 222-483 of human ACOT2 (NP_006812.3).
Immunogen Sequence: IVDMFGTGGGLLEYRASLLA GKGFAVMALAYYNYEDLPKT METLHLEYFEEAMNYLLSHP EVKGPGVGLLGISKGGELCL SMASFLKGITAAVVINGSVA NVGGTLHYKGETLPPVGVNR NRIKVTKDGYADIVDVLNSP LEGPDQKSFIPVERAESTFL FLVGQDDHNWKSEFYANEAC KRLQAHGRRKPQIICYPETG HYIEPPYFPLCRASLHALVG SPIIWGGEPRAHAMAQVDA
Tissue Specificity Strongest expression in heart, liver, muscle and kidney. Weak in placenta and pancreas.
Function Catalyzes the hydrolysis of acyl-CoAs into free fatty acids and coenzyme A (CoASH), regulating their respective intracellular levels. Displays higher activity toward long chain acyl CoAs (C14-C20). The enzyme is involved in enhancing the hepatic fatty acid oxidation in mitochondria.
Protein Name Acyl-Coenzyme A Thioesterase 2 - Mitochondrial
Acyl-Coa Thioesterase 2
Acyl-Coenzyme A Thioester Hydrolase 2a
Cte-Ia
Long-Chain Acyl-Coa Thioesterase 2
Zap128
Database Links Reactome: R-HSA-77289
Reactome: R-HSA-9033241
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Acyl-Coenzyme A Thioesterase 2 - Mitochondrial antibody
Anti-Acyl-Coa Thioesterase 2 antibody
Anti-Acyl-Coenzyme A Thioester Hydrolase 2a antibody
Anti-Cte-Ia antibody
Anti-Long-Chain Acyl-Coa Thioesterase 2 antibody
Anti-Zap128 antibody
Anti-ACOT2 antibody
Anti-PTE2 antibody
Anti-PTE2A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance