• Biotinylated STJ72158 (0. 1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-Aconitase 2, Biotinlyated antibody (Internal) (STJ73181)

SKU:
STJ73181-100

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Host: Goat
Applications: Pep-ELISA/WB/IF/IHC
Reactivity: Human/Mouse/Rat/Dog/Cow/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Aconitase 2, Biotinlyated (Internal) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.1-0.3µg/ml
ELISA-antibody detection limit dilution 1:2000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ACO2
Gene ID: 50
Uniprot ID: ACON_HUMAN
Immunogen Region: Internal
Accession Number: NP_001089.1
Immunogen Sequence: QHVDVSPTSQRLQ.
Function Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.
Protein Name Aconitate Hydratase - Mitochondrial
Aconitase
Citrate Hydro-Lyase
Database Links Reactome: R-HSA-1268020
Reactome: R-HSA-71403
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Aconitate Hydratase - Mitochondrial antibody
Anti-Aconitase antibody
Anti-Citrate Hydro-Lyase antibody
Anti-ACO2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance