• Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-brain, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100
  • Immunohistochemical analysis of paraffin-embedded human-breast, antibody was diluted at 1:100
  • Western blot analysis of JK lysis using Acetyl-Ku-70 (K542) antibody.. Secondary antibody was diluted at 1:20000
  • Western blot analysis of Jurkat cells using Acetyl-Ku-70 (K542) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-XRCC6-Lys542 antibody (STJ90148)

SKU:
STJ90148

Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-X-ray repair cross-complementing protein 6-Lys542 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC-P 1:100-300
ELISA 1:20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: XRCC6
Gene ID: 2547
Uniprot ID: XRCC6_HUMAN
Specificity: Acetyl-Ku-70 (K542) Polyclonal Antibody detects endogenous levels of Ku-70 protein only when acetylated at K542.
Immunogen: Synthesized acetyl-peptide derived from the human Ku-70 around the acetylation site of K542.
Function Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.
Protein Name X-Ray Repair Cross-Complementing Protein 6
5'-Deoxyribose-5-Phosphate Lyase Ku70
5'-Drp Lyase Ku70
70 Kda Subunit Of Ku Antigen
Atp-Dependent Dna Helicase 2 Subunit 1
Atp-Dependent Dna Helicase Ii 70 Kda Subunit
Ctc Box-Binding Factor 75 Kda Subunit
Ctc75
Ctcbf
Dna Repair Protein Xrcc6
Lupus Ku Autoantigen Protein P70
Ku70
Thyroid-Lupus Autoantigen
Tlaa
X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6
Database Links Reactome: R-HSA-164843
Reactome: R-HSA-1834949
Reactome: R-HSA-3270619
Reactome: R-HSA-5693571
Reactome: R-HSA-6798695
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-X-Ray Repair Cross-Complementing Protein 6 antibody
Anti-5'-Deoxyribose-5-Phosphate Lyase Ku70 antibody
Anti-5'-Drp Lyase Ku70 antibody
Anti-70 Kda Subunit Of Ku Antigen antibody
Anti-Atp-Dependent Dna Helicase 2 Subunit 1 antibody
Anti-Atp-Dependent Dna Helicase Ii 70 Kda Subunit antibody
Anti-Ctc Box-Binding Factor 75 Kda Subunit antibody
Anti-Ctc75 antibody
Anti-Ctcbf antibody
Anti-Dna Repair Protein Xrcc6 antibody
Anti-Lupus Ku Autoantigen Protein P70 antibody
Anti-Ku70 antibody
Anti-Thyroid-Lupus Autoantigen antibody
Anti-Tlaa antibody
Anti-X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6 antibody
Anti-XRCC6 antibody
Anti-G22P1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance