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Anti-Acetyl-TUBA1B-4A8 antibody [4A8] (STJ97537)

Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma using a-tubulin (Acetyl Lys40) Mouse mAb diluted at 1:200.
Immunohistochemical analysis of paraffin-embedded Mouse Brain Tissue using a-tubulin (Acetyl Lys40) Mouse mAb diluted at 1:200.
Immunofluorescence analysis of Rat-spinal-cord tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of extracts from Hela cells, untreated (-) or treated with TSA (1 Mu M, 18 hr; +), using Acetyl-a-tubulin (Lys40) Mouse mAb 1:2000.
Immunofluorescence analysis of Mouse-lung tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunofluorescence analysis of Human-liver-cancer tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Immunohistochemical analysis of paraffin-embedded Mouse-heart tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, Alpha-tubulin (Acetyl Lys40) monoclonal antibody (4A8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-Acetyl-TUBA1B-4A8 antibody [4A8] (STJ97537)

SKU:

STJ97537

£46.00 - £226.00

Current Stock:

General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Mouse
Applications:	WB/IF/IHC
Reactivity:	Human/Rat/Mouse/D.melanogaster
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Mouse monoclonal antibody anti-Acetyl-Tubulin alpha-1B chain-4A8 is suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry research applications.

Clonality:	Monoclonal
Clone ID:	4A8
Conjugation:	Unconjugated
Isotype:	IgG1
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:1000-2000 IHC 1:50-100 IF 1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	TUBA1B
Gene ID:	10376
Uniprot ID:	TBA1B_HUMAN
Specificity:	Alpha-tubulin (Acetyl Lys40) protein detects endogenous levels of Alpha-tubulin (Acetyl Lys40)
Immunogen:	Synthetic Peptide of Alpha-tubulin (Acetyl Lys40)

Function	Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Protein Name	Tubulin Alpha-1b Chain Alpha-Tubulin Ubiquitous Tubulin K-Alpha-1 Tubulin Alpha-Ubiquitous Chain Cleaved Into - Detyrosinated Tubulin Alpha-1b Chain
Database Links	Reactome: R-HSA-1445148 Reactome: R-HSA-190840 Reactome: R-HSA-190861 Reactome: R-HSA-2132295 Reactome: R-HSA-2467813 Reactome: R-HSA-2500257 Reactome: R-HSA-3371497 Reactome: R-HSA-380320 Reactome: R-HSA-389960 Reactome: R-HSA-389977 Reactome: R-HSA-437239 Reactome: R-HSA-5610787 Reactome: R-HSA-5617833 Reactome: R-HSA-5620924 Reactome: R-HSA-5626467 Reactome: R-HSA-5663220 Reactome: R-HSA-6807878 Reactome: R-HSA-6811434 Reactome: R-HSA-6811436 Reactome: R-HSA-68877 Reactome: R-HSA-8852276 Reactome: R-HSA-8955332 Reactome: R-HSA-9013407 Reactome: R-HSA-9609690 Reactome: R-HSA-9609736 Reactome: R-HSA-9619483 Reactome: R-HSA-9646399 Reactome: R-HSA-9648025 Reactome: R-HSA-9668328 Reactome: R-HSA-983189
Cellular Localisation	Cytoplasm Cytoskeleton
Alternative Antibody Names	Anti-Tubulin Alpha-1b Chain antibody Anti-Alpha-Tubulin Ubiquitous antibody Anti-Tubulin K-Alpha-1 antibody Anti-Tubulin Alpha-Ubiquitous Chain Cleaved Into - Detyrosinated Tubulin Alpha-1b Chain antibody Anti-TUBA1B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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