• Western blot analysis of HeLa, using Acetyl-Histone H3-K56 rabbit polyclonal antibody (STJ29398) at 1:10000 dilution. HeLa cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of NIH/3T3, using Acetyl-Histone H3-K56 rabbit polyclonal antibody (STJ29398) at 1:10000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of C6, using Acetyl-Histone H3-K56 rabbit polyclonal antibody (STJ29398) at 1:10000 dilution. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of C6 treated by TSA jia C6 cells using Acetyl-Histone H3-K56 rabbit polyclonal antibody (STJ29398) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 treated by TSA jia NIH-3T3 cells using Acetyl-Histone H3-K56 rabbit polyclonal antibody (STJ29398) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U-2 OS treated by TSA jia U-2 OS cells using Acetyl-Histone H3-K56 rabbit polyclonal antibody (STJ29398) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Chromatin immunoprecipitation analysis of extracts of Hale cells, using Acetyl-Histone H3-K56 antibody (STJ29398) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Anti-Acetyl-Histone H3-K56 antibody (STJ29398)

Anti-Acetyl-Histone H3-K56 antibody (STJ29398)

SKU:
STJ29398

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-Histone H3-K56 is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic acetylated peptide around K56 of human Histone H3 (NP_003520.1).
Immunogen Sequence: YQKST
Background This gene encodes a translation initiation factor that is involved in mitochondrial protein synthesis. Polymorphism in this gene is associated with the onset of Parkinson s disease. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 5.

Information sourced from Uniprot.org

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