• Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K5 antibody (STJ118069) at 1:1000 dilution. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hoursSecondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunohistochemistry of paraffin-embedded rat spleen using Acetyl-Histone H2B-K5 antibody (STJ118069) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded human gastric cancer using Acetyl-Histone H2B-K5 antibody (STJ118069) at dilution of 1:100 (40x lens).
  • Immunohistochemistry of paraffin-embedded mouse spleen using Acetyl-Histone H2B-K5 antibody (STJ118069) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K5 antibody (STJ118069) at dilution of 1:100. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K5 antibody (STJ118069) at dilution of 1:100. HeLa cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K5 antibody (STJ118069) at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.

Anti-Acetyl-Histone H2B-K5 antibody (STJ118069)

SKU:
STJ118069

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Current Stock:
Host: Rabbit
Applications: WB, IHC, IF, IP
Reactivity: Human, Mouse, Rat
Note: FOR RESEARCH USE ONLY (RUO).
Short Description: Rabbit polyclonal antibody anti-Acetyl-Histone H2B-K5 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS containing 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:50-1:200
IF 1:50-1:200
IP 1:50-1:100
ChIP 1:50-1:100
Storage Instruction: Store in a freezer at-20°C and avoid freeze-thaw cycles.
Gene Symbol: H2BC21
H2BC5
Gene ID: 8349
3017
Uniprot ID: H2B2E_HUMAN
H2B1D_HUMAN
Immunogen: A synthetic acetylated peptide corresponding to residues surrounding K5 of human Histone H2B
Database Links Reactome: R-HSA-110328:::Reactome: R-HSA-110328
Cellular Localisation Nucleus
Chromosome
Protein Name Histone H2b Type 2-E
H2b-Clustered Histone 21
Histone H2b-Gl105
Histone H2b.q
H2b/Q
Alternative Names Anti-Histone H2b Type 2-E antibody
Anti-H2b-Clustered Histone 21 antibody
Anti-Histone H2b-Gl105 antibody
Anti-Histone H2b.q antibody
Anti-H2b/Q antibody
Anti-H2BC21 antibody
Anti-H2BFQ antibody
Anti-HIST2H2BE antibody

Information sourced from uniprot.org

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