• Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H1. 4-K26 (STJ11103882) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. U2OS cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H1. 4-K26 (STJ11103882) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. NIH/3T3 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using Acetyl-Histone H1. 4-K26 (STJ11103882) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. C6 cells were treated by TSA (1 uM) at 37 °C for 18 hours. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of Acetyl-Histone H1. 4-K26 in paraffin-embedded mouse brain using Acetyl-Histone H1. 4-K26 Rabbit polyclonal antibody (STJ11103882) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Acetyl-Histone H1. 4-K26 in paraffin-embedded human tonsil using Acetyl-Histone H1. 4-K26 Rabbit polyclonal antibody (STJ11103882) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of Acetyl-Histone H1. 4-K26 in paraffin-embedded rat ovary using Acetyl-Histone H1. 4-K26 Rabbit polyclonal antibody (STJ11103882) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-Acetyl-H1-4-K26 antibody (STJ11103882)

SKU:
STJ11103882

£134.50 - £666.50
Current Stock:
Host: Rabbit
Applications: IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Acetyl-Histone H1.4-K26 is suitable for use in Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H1-4
Gene ID: 3008
Uniprot ID: H14_HUMAN
Immunogen: A synthetic acetylated peptide around K26 of human Histone H14 (NP_005312.1).
Immunogen Sequence: ARKSA
Post Translational Modifications H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter. Acetylated at Lys-26. Deacetylated at Lys-26 by SIRT1. Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. ADP-ribosylated on Ser-150 in response to DNA damage.
Function Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.
Protein Name Histone H1.4
Histone H1b
Histone H1s-4
Database Links Reactome: R-HSA-140342
Reactome: R-HSA-2559584
Cellular Localisation Nucleus
Chromosome
Mainly Localizes In Heterochromatin
Dysplays A Punctuate Staining Pattern In The Nucleus
Alternative Antibody Names Anti-Histone H1.4 antibody
Anti-Histone H1b antibody
Anti-Histone H1s-4 antibody
Anti-H1-4 antibody
Anti-H1F4 antibody
Anti-HIST1H1E antibody

Information sourced from Uniprot.org

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