Anti-ACE2 antibody [PT0172R] (STJA0039562)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgGk
General Information
| Short Description | Rabbit monoclonal anti-Angiotensin-Converting Enzyme 2 for use in WB, IHC, IF, IP and ELISA in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | PT0172R |
| Isotype | IgGk |
| Conjugation | Unconjugated |
| Purification | Protein A |
| Dilution Range | IHC 1:1000-1:4000WB 1:1000-1:5000IF 1:200-1:1000ELISA 1:5000-1:20000IP 1:50-1:200 |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ACE2 |
| Gene ID | 59272 |
| Uniprot ID | ACE2_HUMAN |
| Specificity | Endogenous |
Additional Info
| Post Translational Modifications | N-glycosylation on Asn-90 may limit SARS infectivity. Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. Phosphorylated. Phosphorylation at Tyr-781 probably inhibits interaction with AP2M1 and enables interactions with proteins containing SH2 domains. Ubiquitinated. Ubiquitinated on Lys-788 via 'Lys-48'-linked ubiquitin. 'Lys-48'-linked deubiquitinated by USP50 on the Lys-788.leading to its stabilization. |
| Function | Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins (apelin-13, Pyr1apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency. In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity. (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. Isoform 2: Non-functional as a carboxypeptidase. Isoform 2: (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2. |
| Protein Name | Angiotensin-Converting Enzyme 2Angiotensin-Converting Enzyme HomologAcehAngiotensin-Converting Enzyme-Related CarboxypeptidaseAce-Related CarboxypeptidaseMetalloprotease Mprot15 Cleaved Into - Processed Angiotensin-Converting Enzyme 2 |
| Database Links | Reactome: R-HSA-2022377Reactome: R-HSA-9678110Reactome: R-HSA-9679191Reactome: R-HSA-9694614Reactome: R-HSA-9733458 |
| Cellular Localisation | Processed Angiotensin-Converting Enzyme 2: SecretedCell MembraneSingle-Pass Type I Membrane ProteinCytoplasmCell ProjectionCiliumApical Cell MembraneDetected In Both Cell Membrane And Cytoplasm In NeuronsIsoform 2: Apical Cell Membrane |
| Alternative Antibody Names | Anti-Angiotensin-Converting Enzyme 2 antibodyAnti-Angiotensin-Converting Enzyme Homolog antibodyAnti-Aceh antibodyAnti-Angiotensin-Converting Enzyme-Related Carboxypeptidase antibodyAnti-Ace-Related Carboxypeptidase antibodyAnti-Metalloprotease Mprot15 Cleaved Into - Processed Angiotensin-Converting Enzyme 2 antibodyAnti-ACE2 antibodyAnti-UNQ868 antibodyAnti-PRO1885 antibody |
Information sourced from Uniprot.org