• Immunofluorescence analysis of L929 cells using ACBD3 Rabbit polyclonal antibody (STJ118995) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using ACBD3 Rabbit polyclonal antibody (STJ118995) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using ACBD3 Rabbit polyclonal antibody (STJ118995) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ACBD3 antibody (1-160) (STJ118995)

SKU:
STJ118995

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACBD3 (1-160) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACBD3
Gene ID: 64746
Uniprot ID: GCP60_HUMAN
Immunogen Region: 1-160
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ACBD3 (NP_073572.2).
Immunogen Sequence: MAAVLNAERLEVSVDGLTLS PDPEERPGAEGAPLLPPPLP PPSPPGSGRGPGASGEQPEP GEAAAGGAAEEARRLEQRWG FGLEELYGLALRFFKEKDGK AFHPTYEEKLKLVALHKQVL MGPYNPDTCPEVGFFDVLGN DRRREWAALGNMSKEDAMVE
Tissue Specificity Ubiquitous, with highest expression in testis and ovary.
Function Involved in the maintenance of Golgi structure by interacting with giantin, affecting protein transport between the endoplasmic reticulum and Golgi. Involved in hormone-induced steroid biosynthesis in testicular Leydig cells. Recruits PI4KB to the Golgi apparatus membrane.enhances the enzyme activity of PI4KB activity via its membrane recruitment thereby increasing the local concentration of the substrate in the vicinity of the kinase. (Microbial infection) Plays an essential role in Aichi virus RNA replication by recruiting PI4KB at the viral replication sites.
Protein Name Golgi Resident Protein Gcp60
Acyl-Coa-Binding Domain-Containing Protein 3
Golgi Complex-Associated Protein 1
Gocap1
Golgi Phosphoprotein 1
Golph1
Pbr- And Pka-Associated Protein 7
Peripheral Benzodiazepine Receptor-Associated Protein Pap7 Cleaved Into - Golgi Resident Protein Gcp60 - N-Terminally Processed
Database Links Reactome: R-HSA-432722
Cellular Localisation Golgi Apparatus Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Mitochondrion
Also Mitochondrial (Via Its Interaction With Pbr)
Alternative Antibody Names Anti-Golgi Resident Protein Gcp60 antibody
Anti-Acyl-Coa-Binding Domain-Containing Protein 3 antibody
Anti-Golgi Complex-Associated Protein 1 antibody
Anti-Gocap1 antibody
Anti-Golgi Phosphoprotein 1 antibody
Anti-Golph1 antibody
Anti-Pbr- And Pka-Associated Protein 7 antibody
Anti-Peripheral Benzodiazepine Receptor-Associated Protein Pap7 Cleaved Into - Golgi Resident Protein Gcp60 - N-Terminally Processed antibody
Anti-ACBD3 antibody
Anti-GCP60 antibody
Anti-GOCAP1 antibody
Anti-GOLPH1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance