• Western blot analysis of extracts of various cell lines, using ACAT2 antibody (STJ110176) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of L929 cells using ACAT2 antibody (STJ110176) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded rat ovary using ACAT2 antibody (STJ110176) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using ACAT2 antibody (STJ110176) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human colon carcinoma using ACAT2 antibody (STJ110176) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human oophoroma using ACAT2 antibody (STJ110176) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human oophoroma using ACAT2 antibody (STJ110176) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using ACAT2 antibody (STJ110176) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse kidney using ACAT2 antibody (STJ110176) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of paraffin-embedded rat ovary using ACAT2 antibody (STJ110176) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of L929 cells using ACAT2 antibody (STJ110176) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using ACAT2 antibody (STJ110176) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ACAT2 antibody (159-277) (STJ110176)

SKU:
STJ110176

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACAT2 (159-277) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACAT2
Gene ID: 39
Uniprot ID: THIC_HUMAN
Immunogen Region: 159-277
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 159-277 of human ACAT2 (NP_005882.2).
Immunogen Sequence: HMGITAENVAKKWQVSREDQ DKVAVLSQNRTENAQKAGHF DKEIVPVLVSTRKGLIEVKT DEFPRHGSNIEAMSKLKPYF LTDGTGTVTPANASGINDGA AAVVLMKKSEADKRGLTPL
Function Involved in the biosynthetic pathway of cholesterol.
Protein Name Acetyl-Coa Acetyltransferase - Cytosolic
Acetyl-Coa Transferase-Like Protein
Cytosolic Acetoacetyl-Coa Thiolase
Database Links Reactome: R-HSA-191273
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Acetyl-Coa Acetyltransferase - Cytosolic antibody
Anti-Acetyl-Coa Transferase-Like Protein antibody
Anti-Cytosolic Acetoacetyl-Coa Thiolase antibody
Anti-ACAT2 antibody
Anti-ACTL antibody

Information sourced from Uniprot.org

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