Western blot analysis of various lysates using ACAT2 Rabbit pAb (STJ110176) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-ACAT2 antibody (159-277) (STJ110176)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ110176
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General Information

Short DescriptionRabbit polyclonal ACAT2 (159-277) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/IHC-P/IF/ICC/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolACAT2
Gene ID39
Uniprot IDTHIC_HUMAN
Immunogen Region159-277
Immunogen SequenceHMGITAENVAKKWQVSREDQ DKVAVLSQNRTENAQKAGHF DKEIVPVLVSTRKGLIEVKT DEFPRHGSNIEAMSKLKPYF LTDGTGTVTPANASGINDGA AAVVLMKKSEADKRGLTPL
SpecificityA synthetic peptide corresponding to a sequence within amino acids 159-277 of human ACAT2 (NP_005882.2).

Additional Info

Function Involved in the biosynthetic pathway of cholesterol.
Protein Name Acetyl-Coa Acetyltransferase - Cytosolic
Acetyl-Coa Transferase-Like Protein
Cytosolic Acetoacetyl-Coa Thiolase
Database Links Reactome: R-HSA-191273
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Acetyl-Coa Acetyltransferase - Cytosolic antibody
Anti-Acetyl-Coa Transferase-Like Protein antibody
Anti-Cytosolic Acetoacetyl-Coa Thiolase antibody
Anti-ACAT2 antibody
Anti-ACTL antibody

Information sourced from Uniprot.org

Citations

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