• Biotinylated STJ72089 (0. 1µg/ml) staining of Mouse (A) and Rat (B) Liver lysates (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-ACAT1, Biotinylated antibody (253-266) {Biotin} (STJ73206)

SKU:
STJ73206-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IF
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ACAT1, Biotinylated (253-266) is suitable for use in ELISA, Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: ELISA-antibody detection limit dilution 1:4000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ACAT1
Gene ID: 38
Uniprot ID: THIL_HUMAN
Immunogen Region: 253-266
Accession Number: NP_000010.1
Immunogen Sequence: DEEYKRVDFSKVPK.
Function This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism.
Protein Name Acetyl-Coa Acetyltransferase - Mitochondrial
Acetoacetyl-Coa Thiolase
T2
Database Links Reactome: R-HSA-70895
Reactome: R-HSA-77108
Reactome: R-HSA-77111
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Acetyl-Coa Acetyltransferase - Mitochondrial antibody
Anti-Acetoacetyl-Coa Thiolase antibody
Anti-T2 antibody
Anti-ACAT1 antibody
Anti-ACAT antibody
Anti-MAT antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance