• Immunofluorescence analysis of NIH/3T3 cells using ACADM Rabbit monoclonal antibody (STJ11102105) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse kidney using ACADM Rabbit monoclonal antibody (STJ11102105) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon using ACADM Rabbit monoclonal antibody (STJ11102105) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using ACADM Rabbit monoclonal antibody (STJ11102105) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ACADM antibody (200-300) [S5MR] (STJ11102105)

SKU:
STJ11102105

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ACADM (200-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACADM
Gene ID: 34
Uniprot ID: ACADM_HUMAN
Immunogen Region: 200-300
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ACADM (P11310).
Immunogen Sequence: WYFLLARSDPDPKAPANKAF TGFIVEADTPGIQIGRKELN MGQRCSDTRGIVFEDVKVPK ENVLIGDGAGFKVAMGAFDK TRPVVAAGAVGLAQRALDEA T
Post Translational Modifications Acetylated. Could occur at proximity of the cofactor-binding sites and reduce the catalytic activity. Could be deacetylated by SIRT3.
Function Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains.
Protein Name Medium-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial
Mcad
Medium Chain Acyl-Coa Dehydrogenase
Mcadh
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-77288
Reactome: R-HSA-77346
Reactome: R-HSA-77348
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Medium-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial antibody
Anti-Mcad antibody
Anti-Medium Chain Acyl-Coa Dehydrogenase antibody
Anti-Mcadh antibody
Anti-ACADM antibody

Information sourced from Uniprot.org

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