Anti-ACADM antibody (200-300) [S5MR] (STJ11102105)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal ACADM (200-300) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S5MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:1000-1:6000IHC-P:1:200-1:800IF/ICC:1:100-1:400ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ACADM |
| Gene ID | 34 |
| Uniprot ID | ACADM_HUMAN |
| Immunogen Region | 200-300 |
| Immunogen Sequence | WYFLLARSDPDPKAPANKAF TGFIVEADTPGIQIGRKELN MGQRCSDTRGIVFEDVKVPK ENVLIGDGAGFKVAMGAFDK TRPVVAAGAVGLAQRALDEA T |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 200-300 of human ACADM (P11310). |
Additional Info
| Post Translational Modifications | Acetylated. Could occur at proximity of the cofactor-binding sites and reduce the catalytic activity. Could be deacetylated by SIRT3. |
| Function | Medium-chain specific acyl-CoA dehydrogenase is one of the acyl-CoA dehydrogenases that catalyze the first step of mitochondrial fatty acid beta-oxidation, an aerobic process breaking down fatty acids into acetyl-CoA and allowing the production of energy from fats. The first step of fatty acid beta-oxidation consists in the removal of one hydrogen from C-2 and C-3 of the straight-chain fatty acyl-CoA thioester, resulting in the formation of trans-2-enoyl-CoA. Electron transfer flavoprotein (ETF) is the electron acceptor that transfers electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase). Among the different mitochondrial acyl-CoA dehydrogenases, medium-chain specific acyl-CoA dehydrogenase acts specifically on acyl-CoAs with saturated 6 to 12 carbons long primary chains. |
| Protein Name | Medium-Chain Specific Acyl-Coa Dehydrogenase - MitochondrialMcadMedium Chain Acyl-Coa DehydrogenaseMcadh |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-77288Reactome: R-HSA-77346Reactome: R-HSA-77348 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Medium-Chain Specific Acyl-Coa Dehydrogenase - Mitochondrial antibodyAnti-Mcad antibodyAnti-Medium Chain Acyl-Coa Dehydrogenase antibodyAnti-Mcadh antibodyAnti-ACADM antibody |
Information sourced from Uniprot.org