• Immunofluorescence staining of methanol-fixed Hela cells using ABL2 mouse mAb showing cytoplasm localization using Arg monoclonal antibody.
  • Western blot analysis using Arg monoclonal antibody against truncated ABL2 recombinant protein.

Anti-ABL2 antibody [1H1B11] (STJ97847)

SKU:
STJ97847

Current Stock:
Host: Mouse
Applications: WB/IF/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Tyrosine-protein kinase ABL2 is suitable for use in Western Blot, Immunofluorescence and ELISA research applications.
Clonality: Monoclonal
Clone ID: 1H1B11
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABL2
Gene ID: 27
Uniprot ID: ABL2_HUMAN
Specificity: Arg Monoclonal Antibody detects endogenous levels of Arg protein.
Immunogen: Purified recombinant fragment of Arg expressed in E. Coli.
Post Translational Modifications Phosphorylated at Tyr-261 by ABL1 in response to oxidative stress. Phosphorylated by PDGFRB. Polyubiquitinated. Polyubiquitination of ABL2 leads to degradation.
Function Non-receptor tyrosine-protein kinase that plays an ABL1-overlapping role in key processes linked to cell growth and survival such as cytoskeleton remodeling in response to extracellular stimuli, cell motility and adhesion and receptor endocytosis. Coordinates actin remodeling through tyrosine phosphorylation of proteins controlling cytoskeleton dynamics like MYH10 (involved in movement).CTTN (involved in signaling).or TUBA1 and TUBB (microtubule subunits). Binds directly F-actin and regulates actin cytoskeletal structure through its F-actin-bundling activity. Involved in the regulation of cell adhesion and motility through phosphorylation of key regulators of these processes such as CRK, CRKL, DOK1 or ARHGAP35. Adhesion-dependent phosphorylation of ARHGAP35 promotes its association with RASA1, resulting in recruitment of ARHGAP35 to the cell periphery where it inhibits RHO. Phosphorylates multiple receptor tyrosine kinases like PDGFRB and other substrates which are involved in endocytosis regulation such as RIN1. In brain, may regulate neurotransmission by phosphorylating proteins at the synapse. ABL2 acts also as a regulator of multiple pathological signaling cascades during infection. Pathogens can highjack ABL2 kinase signaling to reorganize the host actin cytoskeleton for multiple purposes, like facilitating intracellular movement and host cell exit. Finally, functions as its own regulator through autocatalytic activity as well as through phosphorylation of its inhibitor, ABI1. Positively regulates chemokine-mediated T-cell migration, polarization, and homing to lymph nodes and immune-challenged tissues, potentially via activation of NEDD9/HEF1 and RAP1.
Protein Name Tyrosine-Protein Kinase Abl2
Abelson Murine Leukemia Viral Oncogene Homolog 2
Abelson Tyrosine-Protein Kinase 2
Abelson-Related Gene Protein
Tyrosine-Protein Kinase Arg
Database Links Reactome: R-HSA-428890
Reactome: R-HSA-9013149
Reactome: R-HSA-9013423
Reactome: R-HSA-9706369
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Tyrosine-Protein Kinase Abl2 antibody
Anti-Abelson Murine Leukemia Viral Oncogene Homolog 2 antibody
Anti-Abelson Tyrosine-Protein Kinase 2 antibody
Anti-Abelson-Related Gene Protein antibody
Anti-Tyrosine-Protein Kinase Arg antibody
Anti-ABL2 antibody
Anti-ABLL antibody
Anti-ARG antibody

Information sourced from Uniprot.org

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