• Biotinylated STJ71633 (1µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-ABCD3, Biotinylated antibody (Internal) {Biotin} (STJ73239)

SKU:
STJ73239-100

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Host: Goat
Applications: Pep-ELISA/WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-ABCD3, Biotinylated (Internal) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Biotin
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: ABCD3
Gene ID: 5825
Uniprot ID: ABCD3_HUMAN
Immunogen Region: Internal
Accession Number: NP_002849.1
Specificity: This antibody is expected to recognize one isoform (NP_002849.1) only.
Immunogen Sequence: PDGREDQKRKGISD.
Function Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that catalyzes the transport of long-chain fatty acids (LCFA)-CoA, dicarboxylic acids-CoA, long-branched-chain fatty acids-CoA and bile acids from the cytosol to the peroxisome lumen for beta-oxydation. Has fatty acyl-CoA thioesterase and ATPase activities. Probably hydrolyzes fatty acyl-CoAs into free fatty acids prior to their ATP-dependent transport into peroxisomes. Thus, play a role in regulation of LCFAs and energy metabolism namely, in the degradation and biosynthesis of fatty acids by beta-oxidation.
Protein Name Atp-Binding Cassette Sub-Family D Member 3
70 Kda Peroxisomal Membrane Protein
Pmp70
Database Links Reactome: R-HSA-1369062
Reactome: R-HSA-8980692
Reactome: R-HSA-9013106
Reactome: R-HSA-9603798
Cellular Localisation Peroxisome Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Atp-Binding Cassette Sub-Family D Member 3 antibody
Anti-70 Kda Peroxisomal Membrane Protein antibody
Anti-Pmp70 antibody
Anti-ABCD3 antibody
Anti-PMP70 antibody
Anti-PXMP1 antibody

Information sourced from Uniprot.org

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