• Western blot analysis of extracts of mouse liver, using ABCB11 antibody (STJ110765) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Immunofluorescence analysis of NIH-3T3 cells using ABCB11 Polyclonal Antibody (STJ110765) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using ABCB11 antibody (STJ110765) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ABCB11 antibody (1050-1150) (STJ110765)

SKU:
STJ110765

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ABCB11 (1050-1150) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ABCB11
Gene ID: 8647
Uniprot ID: ABCBB_HUMAN
Immunogen Region: 1050-1150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1050-1150 of human ABCB11 (NP_003733.2).
Immunogen Sequence: RFFQLLDRQPPISVYNTAGE KWDNFQGKIDFVDCKFTYPS RPDSQVLNGLSVSISPGQTL AFVGSSGCGKSTSIQLLERF YDPDQGKVMIDGHDSKKVNV Q
Tissue Specificity Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.
Post Translational Modifications N-glycosylated. Ubiquitinated.short-chain ubiquitination regulates cell-Surface expression of ABCB11.
Function Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner. Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts. Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion.
Protein Name Bile Salt Export Pump
Atp-Binding Cassette Sub-Family B Member 11
Database Links Reactome: R-HSA-159418
Reactome: R-HSA-193368
Reactome: R-HSA-5678520
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Recycling Endosome Membrane
Endosome
Cell Membrane
Internalized At The Canalicular Membrane Through Interaction With The Adapter Protein Complex 2 (Ap-2)
At Steady State
Localizes In The Canalicular Membrane But Is Also Present In Recycling Endosomes
Abcb11 Constantly And Rapidly Exchanges Between The Two Sites Through Tubulo-Vesicles Carriers That Move Along Microtubules
Microtubule-Dependent Trafficking Of Abcb11 Is Enhanced By Taurocholate And Camp And Regulated By Stk11 Through A Pka-Mediated Pathway
Trafficking Of Newly Synthesized Abcb11 Through Endosomal Compartment To The Bile Canalicular Membrane Is Accelerated By Camp But Not By Taurocholate
Cell Membrane Expression Is Up-Regulated By Short- And Medium-Chain Fatty Acids
Alternative Antibody Names Anti-Bile Salt Export Pump antibody
Anti-Atp-Binding Cassette Sub-Family B Member 11 antibody
Anti-ABCB11 antibody
Anti-BSEP antibody

Information sourced from Uniprot.org

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