| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | ABCB11 |
| Gene ID: | 8647 |
| Uniprot ID: | ABCBB_HUMAN |
| Immunogen Region: | 1050-1150 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 1050-1150 of human ABCB11 (NP_003733.2). |
| Immunogen Sequence: | RFFQLLDRQPPISVYNTAGE KWDNFQGKIDFVDCKFTYPS RPDSQVLNGLSVSISPGQTL AFVGSSGCGKSTSIQLLERF YDPDQGKVMIDGHDSKKVNV Q |
| Tissue Specificity | Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ. |
| Post Translational Modifications | N-glycosylated. Ubiquitinated.short-chain ubiquitination regulates cell-Surface expression of ABCB11. |
| Function | Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner. Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts. Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion. |
| Protein Name | Bile Salt Export Pump Atp-Binding Cassette Sub-Family B Member 11 |
| Database Links | Reactome: R-HSA-159418 Reactome: R-HSA-193368 Reactome: R-HSA-5678520 |
| Cellular Localisation | Apical Cell Membrane Multi-Pass Membrane Protein Recycling Endosome Membrane Endosome Cell Membrane Internalized At The Canalicular Membrane Through Interaction With The Adapter Protein Complex 2 (Ap-2) At Steady State Localizes In The Canalicular Membrane But Is Also Present In Recycling Endosomes Abcb11 Constantly And Rapidly Exchanges Between The Two Sites Through Tubulo-Vesicles Carriers That Move Along Microtubules Microtubule-Dependent Trafficking Of Abcb11 Is Enhanced By Taurocholate And Camp And Regulated By Stk11 Through A Pka-Mediated Pathway Trafficking Of Newly Synthesized Abcb11 Through Endosomal Compartment To The Bile Canalicular Membrane Is Accelerated By Camp But Not By Taurocholate Cell Membrane Expression Is Up-Regulated By Short- And Medium-Chain Fatty Acids |
| Alternative Antibody Names | Anti-Bile Salt Export Pump antibody Anti-Atp-Binding Cassette Sub-Family B Member 11 antibody Anti-ABCB11 antibody Anti-BSEP antibody |
Information sourced from Uniprot.org