Anti-ABCA7 antibody (1050-1100 aa) (STJ13100648)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 1050-1100 of human ABCA7 conjugated to blue carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-ABCA7 (1050-1100 aa) for use in IHC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | ABCA7 |
| Gene ID | 10347 |
| Uniprot ID | ABCA7_HUMAN |
| Immunogen | A synthetic peptide from aa region 1050-1100 of human ABCA7 conjugated to blue carrier protein was used as the antigen. |
| Immunogen Region | 1050-1100 aa |
| Specificity | Specific for ABCA7. |
Additional Info
| Post Translational Modifications | N-glycosylated. |
| Function | Catalyzes the translocation of specific phospholipids from the cytoplasmic to the extracellular/lumenal leaflet of membrane coupled to the hydrolysis of ATP. Transports preferentially phosphatidylserine over phosphatidylcholine. Plays a role in lipid homeostasis and macrophage-mediated phagocytosis. Binds APOA1 and may function in apolipoprotein-mediated phospholipid efflux from cells. May also mediate cholesterol efflux. May regulate cellular ceramide homeostasis during keratinocyte differentiation. Involved in lipid raft organization and CD1D localization on thymocytes and antigen-presenting cells, which plays an important role in natural killer T-cell development and activation. Plays a role in phagocytosis of apoptotic cells by macrophages. Macrophage phagocytosis is stimulated by APOA1 or APOA2, probably by stabilization of ABCA7. Also involved in phagocytic clearance of amyloid-beta by microglia cells and macrophages. Further limits amyloid-beta production by playing a role in the regulation of amyloid-beta A4 precursor protein (APP) endocytosis and/or processing. Amyloid-beta is the main component of amyloid plaques found in the brains of Alzheimer patients. |
| Protein Name | Phospholipid-Transporting Atpase Abca7Abca-SsnAtp-Binding Cassette Sub-Family A Member 7Autoantigen Ss-NMacrophage Abc Transporter |
| Database Links | Reactome: R-HSA-1369062 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneEarly Endosome MembraneCytoplasmCell ProjectionRuffle MembranePhagocytic CupLocalizes To Cell Membrane Ruffles And Phagocytic Cups Of Macrophages Stimulated With C1q Or Apoptotic CellsLocalizes To The Cytoplasm Of Resting MacrophagesProbably In Golgi And EndosomesLocalizes To The Apical Brush Border Of Cells In The Proximal Tubules Of KidneyIsoform 2: CytoplasmEndoplasmic ReticulumMay Localize To The Endoplasmic Reticulum |
| Alternative Antibody Names | Anti-Phospholipid-Transporting Atpase Abca7 antibodyAnti-Abca-Ssn antibodyAnti-Atp-Binding Cassette Sub-Family A Member 7 antibodyAnti-Autoantigen Ss-N antibodyAnti-Macrophage Abc Transporter antibodyAnti-ABCA7 antibody |
Information sourced from Uniprot.org