Anti-ABCA4 antibody (110-300 aa) (STJ11105939)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ABCA4 (110-300 aa) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:100-1:500ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ABCA4 |
| Gene ID | 24 |
| Uniprot ID | ABCA4_HUMAN |
| Immunogen Region | 110-300 aa |
| Immunogen Sequence | QELLMNAPESQHLGRIWTEL HILSQFMDTLRTHPERIAGR GIRIRDILKDEETLTLFLIK NIGLSDSVVYLLINSQVRPE QFAHGVPDLALKDIACSEAL LERFIIFSQRRGAKTVRYAL CSLSQGTLQWIEDTLYANVD FFKLFRVLPTLLDSRSQGIN LRSWGGILSDMSPRIQEFIH RPSMQDLLWVT |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 110-300 of human ABCA4 (NP_000341.2). |
Additional Info
| Tissue Specificity | Retinal-specific. Seems to be exclusively found in the rims of rod photoreceptor cells. |
| Post Translational Modifications | Proteolytic cleavage by trypsin leads to a 120-kDa N-terminal fragment and a 115-kDa C-terminal fragment that are linked through disulfide bonds. N-glycosylated. Phosphorylation is independent of light exposure and modulates ATPase activity. |
| Function | Flippase that catalyzes in an ATP-dependent manner the transport of retinal-phosphatidylethanolamine conjugates like 11-cis and all-trans isomers of N-retinylidene-phosphatidylethanolamine (N-Ret-PE) from the lumen to the cytoplasmic leaflet of photoreceptor outer segment disk membranes, where 11-cis-retinylidene-phosphatidylethanolamine is then isomerized to its all-trans isomer and reduced by RDH8 to produce all-trans-retinol. This transport activity ensures that all-trans-retinal generated from photoexcitation and 11-cis-retinal not needed for the regeneration of rhodopsin and cone opsins are effectively cleared from the photoreceptors, therefore preventing their accumulation and the formation of toxic bisretinoid. Displays ATPase activity in vitro in absence of retinal substrate. May display GTPase activity that is strongly influenced by the lipid environment and the presence of retinoid compounds. Binds the unprotonated form of N-retinylidene-phosphatidylethanolamine with high affinity in the absence of ATP, and ATP binding and hydrolysis induce a protein conformational change that causes N-retinylidene-phosphatidylethanolamine release. |
| Protein Name | Retinal-Specific Phospholipid-Transporting Atpase Abca4Atp-Binding Cassette Sub-Family A Member 4Rim Abc TransporterRim ProteinvRmpRetinal-Specific Atp-Binding Cassette TransporterStargardt Disease Protein |
| Database Links | Reactome: R-HSA-2453902Reactome: R-HSA-382556Reactome: R-HSA-9918454 |
| Cellular Localisation | MembraneMulti-Pass Membrane ProteinEndoplasmic ReticulumCytoplasmic VesicleCell ProjectionCiliumPhotoreceptor Outer SegmentLocalized To The Rim And Incisures Of Rod Outer Segments Disks |
| Alternative Antibody Names | Anti-Retinal-Specific Phospholipid-Transporting Atpase Abca4 antibodyAnti-Atp-Binding Cassette Sub-Family A Member 4 antibodyAnti-Rim Abc Transporter antibodyAnti-Rim Proteinv antibodyAnti-Rmp antibodyAnti-Retinal-Specific Atp-Binding Cassette Transporter antibodyAnti-Stargardt Disease Protein antibodyAnti-ABCA4 antibodyAnti-ABCR antibody |
Information sourced from Uniprot.org