Anti-ABCA3 antibody (450-530 aa) (STJ190577)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 450-530
General Information
| Short Description | Rabbit polyclonal anti-Phospholipid-transporting ATPase ABCA3 (450-530 aa) for use in WB and ELISA in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ABCA3 |
| Gene ID | 21 |
| Uniprot ID | ABCA3_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 450-530 |
| Immunogen Region | 450-530 aa |
| Specificity | ABCA3 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | N-glycosylated. Localization at intracellular vesicles is accompanied by processing of oligosaccharide from high mannose type to complex type. N-linked glycosylation at Asn-124 and Asn-140 is required for stability and efficient anterograde trafficking and prevents from proteasomal degradation. Proteolytically cleaved by CTSL and to a lower extent by CTSB within multivesicular bodies (MVB) and lamellar bodies (LB) leading to a mature form of 150 kDa. |
| Function | Catalyzes the ATP-dependent transport of phospholipids such as phosphatidylcholine and phosphoglycerol from the cytoplasm into the lumen side of lamellar bodies, in turn participates in the lamellar bodies biogenesis and homeostasis of pulmonary surfactant. Transports preferentially phosphatidylcholine containing short acyl chains. In addition plays a role as an efflux transporter of miltefosine across macrophage membranes and free cholesterol (FC) through intralumenal vesicles by removing FC from the cell as a component of surfactant and protects cells from free cholesterol toxicity. |
| Protein Name | Phospholipid-Transporting Atpase Abca3Abc-C TransporterAtp-Binding Cassette Sub-Family A Member 3Atp-Binding Cassette Transporter 3Atp-Binding Cassette 3Xenobiotic-Transporting Atpase Abca3 Cleaved Into - 150 Kda Mature Form |
| Database Links | Reactome: R-HSA-1369062Reactome: R-HSA-5683678Reactome: R-HSA-5683826Reactome: R-HSA-5688399 |
| Cellular Localisation | EndosomeMultivesicular Body MembraneMulti-Pass Membrane ProteinCytoplasmic Vesicle MembraneLate Endosome MembraneLysosome MembraneLocalized In The Limiting Membrane Of Lamellar Bodies In Lung Alveolar Type Ii CellsTrafficks Via The GolgiSorting Vesicles (Svs) And Late Endosome/Multivesicular Body Network Directly To The Outer Membrane Of Lamellar Bodies In At2 Lung Epithelial Cells Or To Lysosomes And Lysosomal-Related Organelles (Lros) In Other Cells Where Undergoes Proteolytic Cleavage And Oligosaccharide Processing From High Mannose Type To Complex TypeOligomers Formation Takes Place In A Post-Endoplasmic Reticulum Compartment |
| Alternative Antibody Names | Anti-Phospholipid-Transporting Atpase Abca3 antibodyAnti-Abc-C Transporter antibodyAnti-Atp-Binding Cassette Sub-Family A Member 3 antibodyAnti-Atp-Binding Cassette Transporter 3 antibodyAnti-Atp-Binding Cassette 3 antibodyAnti-Xenobiotic-Transporting Atpase Abca3 Cleaved Into - 150 Kda Mature Form antibodyAnti-ABCA3 antibodyAnti-ABC3 antibody |
Information sourced from Uniprot.org